ABS1633M

Anti-IST1 Homolog

serum, from rabbit

• 同義詞: hIST1, Putative MAPK-activating protein PM28
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human
• 物種活性（以同源性預測）: chimpanzee (based on 100% sequence homology)
• 技術: immunofluorescence: suitable; western blot: suitable
• 同型: IgG
• NCBI登錄號: NP_001257904
• UniProt登錄號: P53990
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... IST1(9798)

描述

一般說明

IST1 homolog (UniProt: P53990; also known as hIST1, Putative MAPK-activating protein PM28) is encoded by the IST1 (also known as KIAA0174) gene (Gene ID: 9798) in human. IST1 is an ESCRT-III-like protein that is involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis. IST1 is also involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, it is involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. It is shown to regulate early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST. Six isoforms of IST1 homolog have been described that are generated by alternative splicing.

特異性

This rabbit polyclonal antibody detects IST1 homolog in human cells. It targets an epitope within the first 189 amino acids from the N-terminal half.

免疫原

A recombinant fragment corresponding to the first 189 amino acids from the N-terminal half of human IST1 homolog.

應用

Anti-IST1 Homolog, Cat. No. ABS1633,is a rabbit polyclonal antibody that detects IST1 homolog and has been tested for use in Immunofluorescence and Western Blotting.

Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected IST1 Homolog in 10 µg of HeLa cell lysate.

Western Blotting Analysis: A representative lot detected IST1 Homolog in Western Blotting applications (Bajorek, M., et. al. (2009). Nat Struct Mol Biol. 16(7):754-62; Bajorek, M., et. al. (2009). Mol Biol Cell. 20(5):1360-73).

Immunofluorescence Analysis: A representative lot detected IST1 Homolog in Immunofluorescence applications (Bajorek, M., et. al. (2009). Mol Biol Cell. 20(5):1360-73).

品質

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected IST1 Homolog in 10 µg of Raji cell lysate.

標靶描述

~50 kDa observed; 39.75 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).

儲存和穩定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable