ABN2261

Anti-GAS2L1

from rabbit

• 同義詞: GAS2-like protein 1, GAS2-related protein on chromosome 22, Growth arrest-specific protein 2-like 1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human
• 包裝: antibody small pack of 25 μg
• 技術: immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG
• NCBI登錄號: NP_689422
• UniProt登錄號: Q99501-1
• 目標翻譯後修改: unmodified
• 基因資訊: human ... GAS2L1(10634)

描述

一般說明

GAS2-like protein 1 (UniProt: Q99501-1; also known as GAS2-related protein on chromosome 22, Growth arrest-specific protein 2-like 1, GAS2L1) is encoded by the GAS2L1 (also known as GAR22) gene (Gene ID: 10634) in human. GAS2L1 belongs to the growth-arrest-specific 2 (GAS2) family, whose members mediate the crosstalk between filamentous actin (F-actin) and microtubules. Three members of this family, known as GAS2L1, GAS2L2, and GAS2L3, are shown to be differentially involved in mediating the crosstalk between F-actin and microtubules. GAS2L1 is expressed in testis and brain, GAS2L2 is exclusively expressed in skeletal muscle, and GAS2L3 is found in many different cell types. Although they all localize to actin and microtubules, only the exogenous expression of GAD2L1 and GAS2L2 affect microtubule stability, dynamics, and guidance along actin stress fibers. GAS2L1 is reported to associate with the proximal end of mature centrioles and participates in centriole dynamics and centrosome disjunction. Loss of GAS2L1 can inhibit centrosome disjunction in the G2 phase. On the other hand, overexpression of GAS2L1 can induce premature centrosome separation, which also requires its association with actin, microtubules, and the microtubule end-binding proteins. (Ref.: Stroud MJ et al. (2014). J. Cell Sci. 127(12); 2672-2682; AU, FKC et al. (2017). Dev. Cell 40(1); 81-94).

特異性

This rabbit polyclonal antibody GAS2-like protein 1 (GAS2L1) in human cells. It targets an epitope with in 188 amino acids from the C-terminal half.

免疫原

Epitope: C-terminus

GST-tagged recombinant fragment corresponding to 188 amino acids from the C-terminal region of human GAS2-Like protein 1.

應用

Anti-GAS2L1, Cat. No. ABN2261, is a highly specific rabbit polyclonal antibody that targets GAS2-like protein 1 (Gas2L1) and has been tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A representative lot detected GAS2L1 in Western Blotting applications (Au, F.K., et. al. (2017). Dev Cell. 40(1):81-94).

Immunoprecipitation Analysis: 1 µg from a representative lot detected GAS2L1 in U2OS cell lysate (Courtesy of Dr Robert Z. Qi and Dr. Franco K.C. Au at Hong Kong University of Science and Technology).

Immunofluorescence Analysis: A representative lot detected GAS2L1 in Immunofluorescence applications (Au, F.K., et. al. (2017). Dev Cell. 40(1):81-94).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GAS2L1 in lysate from HeLa cells, but not in lysate from GAS2L1 knockout cells (Courtesy of Dr Robert Z. Qi and Dr. Franco K.C. Au at Hong Kong University of Science and Technology).

品質

Evaluated by Western Blotting in HeLa and GAS2L1 knockout lysate.

Western Blotting Analysis: 0.9 µg/mL of this antibody detected GAS2-like protein 1 (Gas2L1) in HeLa cell lysate. It did not detect GAS2L1 in lysate from knockout cells.

標靶描述

~78 kDa observed; 72.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 10% glycerol.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.