ABN2242

Anti-Vangl2

from rabbit, purified by affinity chromatography

• 同義詞: Vang-like protein 2 (UniProt: Q9ULK5, also known as Loop-tail protein 1 homolog, Strabismus 1, Van Gogh-like protein 2
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human, mouse
• 物種活性（以同源性預測）: bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
• 包裝: antibody small pack of 25 μg
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI登錄號: NP_065068
• UniProt登錄號: Q9ULK5
• 目標翻譯後修改: unmodified
• 基因資訊: human ... VANGL2(57216)

描述

一般說明

Vang-like protein 2 (UniProt: Q9ULK5; also known as Loop-tail protein 1 homolog, Strabismus 1, Van Gogh-like protein 2) is encoded by the VANGL2 (also known as KIAA1215, STB1) gene (Gene ID: 57216) in human. Vangl2 is a homodimeric multi-pass membrane protein that plays a role in the control of early morphogenesis, patterning of both axial midline structures and the development of neural plate. It can also form heterodimer with VANGL1. It is required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process. Vang-like protein 2 is reported to bind to Dvl to cell-autonomously promote efficient Dvl plasma membrane recruitment, a pre-requisite for planar cell polarity activation. At the same time, it can inhibit Dvl from interacting with its downstream effector (Dishevelled associated activator of morphogenesis 1 (Daam1), and functionally suppresses Dvl - Daam1 cascade. Mutations in VANGL2 gene cause neural tube defects that results in defective neural tube closure during the first trimester of pregnancy. (Ref.: Seo, HS et al. (2017). Hum. Mol. Genet. 26(11); 2053-2061).

特異性

This rabbit polyclonal antibody detects Vang-like protein 2 in human and murine cells. It targets an epitope with in 15 amino acids from the N-terminal region.

免疫原

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Vang-like Protein 2.

應用

Anti-Vangl2, Cat. No. ABN2242, is a highly specific rabbit polyclonal antibody that targets Vang-like protein 2 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vangl2 in HT1080 cells.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Vangl2 in human tonsil and mouse embryonic spinal cord tissue sections.

Research Category
Neuroscience

品質

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Vangl2 in HepG2 cell lysate.

標靶描述

~60 kDa observed. Uncharacterized bands may be observed in some lysate(s).

外觀

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1