ABN1676M

Anti-Calcipressin-1

serum, from rabbit

• 同義詞: RCAN-1, Down syndrome critical region protein 1 homolog, Myocyte-enriched calcineurin-interacting protein 1, MCIP1, Regulator of calcineurin 1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 無性繁殖: polyclonal
• application: ICC; WB
• 物種活性: rat, human
• 技術: immunocytochemistry: suitable; western blot: suitable

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: rat, human
• 包裝: antibody small pack of 25 μL
• 技術: immunocytochemistry: suitable; western blot: suitable
• 同型: IgG
• NCBI登錄號: NP_714946
• UniProt登錄號: Q6IN33
• 目標翻譯後修改: unmodified
• 基因資訊: rat ... Rcan1(266766)

描述

一般說明

Calcipressin-1 (Uniprot: Q6IN33; also known as Down syndrome critical region protein 1 homolog, Myocyte-enriched calcineurin-interacting protein 1, MCIP1, Regulator of calcineurin 1, RCAN-1) is encoded by the Rcan1 (also known as Dscr1) gene (Gene ID: 266766) in rat. Calcipressin-1 is a cytoplasmic protein that directly binds to the catalytic domain of calcineurin and acts as its negative regulator. When phosphorylated at the conserved FLISPP motif its capacity to inhibit calcineurin is increased. This phosphorylation also controls the half-life of calcipressin-1 by accelerating its degradation. It is a calcium-responsive enzyme, which is reported to dephosphorylate the nuclear factor of activated T cells (NFAT), which promotes its nuclear translocation and links calcium signaling to transcriptional regulation. Calcipressin-1 displays a quick turnover with a half-life of less than 3 hours. However, a Ser to Ala mutation at Ser163 prolongs its half-life to more than 9 hours. (Ref.: Genesca, L et al. (2003). Biochem. J. 374(2); 567-575).

特異性

This rabbit polyclonal antibody detects calcipressin-1 (RCAN-1) in human and rat cells. It targets an epitope with in 11 amino acids from the C-terminal half.

免疫原

A linear peptide corresponding to 11 amino acids from the C-terminal half of rat Calcipressin-1.

Epitope: unknown

應用

Anti-Calcipressin-1 Antibody, Cat. No. ABN1676, is a highly specific rabbit polyclonal antibody that targets Calcipressin-1 and has been tested in Immunocytochemistry and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A representative lot detected Calcipressin-1 in cultured rat sympathetic neurons (Patel, A., et. al. (2015). Nat Commun. 6:10119).

Immunocytochemistry Analysis: A representative lot detected Calcipressin-1 in sympathetic neurons (Patel, A., et. al. (2015). Nat Commun. 6:10119).

品質

Evaluated by Immunocytochemistry in SH-SY5Y cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Calcipressin-1 in SH-SY5Y cells.

標靶描述

22.95 kDa calculated.

外觀

Unpurified

Rabbit polyclonal antiserum with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable