推薦產品
生物源
rabbit
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
rat, human, mouse
物種活性（以同源性預測）
canine (based on 100% sequence homology), bovine (based on 100% sequence homology)
技術
immunocytochemistry: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... NELFE(7936)
一般說明
NELF-E (Negative elongation factor E) is a main component of NELF human transcription factor complex which associates with DRB sensitivity-inducing factor to stifle transcriptional elongation by RNA polymerase II. NELF-E is an RNA-binding protein that includes arginine-aspartic acid dipeptide repeats. NELF-E RNA-binding activity is essential for NELF to function.
免疫原
KLH-conjugated linear peptide corresponding to human NELF-E.
應用
Anti-NELF-E Antibody is a Rabbit Polyclonal Antibody for detection of NELF-E also known as Negative elongation factor E, NELF-E, RNA-binding protein RD & has been validated in ICC & WB.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected NELF-E in NIH/3T3 and HeLa cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
品質
Evaluated by Western Blot in L6 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected NELF-E on 10 µg of L6 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected NELF-E on 10 µg of L6 cell lysate.
標靶描述
~ 43 kDa observed. Uncharacterized bands may be due to post-translational modification.
外觀
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
分析報告
Control
L6 cell lysate
L6 cell lysate
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes.
Stadelmayer, B; Micas, G; Gamot, A; Martin, P; Malirat, N; Koval, S; Raffel, R; Sobhian et al.
Nature Communications null
Seychelle M Vos et al.
eLife, 5 (2016-06-11)
Transcription regulation in metazoans often involves promoter-proximal pausing of RNA polymerase (Pol) II, which requires the 4-subunit negative elongation factor (NELF). Here we discern the functional architecture of human NELF through X-ray crystallography, protein crosslinking, biochemical assays, and RNA crosslinking
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