ABE1302

Anti-Hec1 Antibody

from rabbit, purified by affinity chromatography

• 同義詞: Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, HsHec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC, Hec1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 無性繁殖: polyclonal
• application: ICC; WB
• 物種活性: human
• 技術: immunocytochemistry: suitable; western blot: suitable

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 物種活性（以同源性預測）: rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• 技術: immunocytochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_006092
• UniProt登錄號: O14777
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... NDC80(10403)

描述

一般說明

The protein HEC1 (highly expressed in cancer 1) is an essential component of the vital NDC80 kinetochore-associated protein complex. The NDC80 complex is required for chromosome segregation and spindle checkpoint activity. HEC1 is essential for proper kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. HEC1 is expressed by all dividing cells and is particularly high in cancers. HEC1 localizes to kinetochores from late prophase to anaphase, specifically at the outer plate of the kinetochore. HEC1 is normally phosphorylated during its active phase at multiple sites and the phosphorylation appears to tune kinetochore-microtubule binding affinity during mitosis. Moreover, recent research using small molecule inhibitors to HEC1′s kinase, Nek2, demonstrate that phosphorylation is required for proper HEC1 function.

免疫原

Epitope: Nuclear localization region

KLH-conjugated linear peptide corresponding to the Nuclear localization region of human Hec1.

應用

Anti-Hec1 Antibody is an antibody against Hec1 Antibody for use in Western Blotting, Immunocytochemistry.

Immunocytochemistry Analysis: 1.0 µg/mL from a representative lot detected Hec1 in HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

品質

Evaluated by Western Blotting in SK-OV-3 cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected Hec1 in 10 µg of SK-OV-3 cell lysate.

標靶描述

~78 kDa observed. Uncharacterized band(s) may appear in some lysates.

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable