ABE1068

Anti-Lin-28B Antibody

from rabbit, purified by affinity chromatography

• 同義詞: Protein lin-28 homolog B, Lin-28B
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 物種活性（以同源性預測）: bovine (based on 100% sequence homology), porcine (based on 100% sequence homology)
• 技術: immunohistochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_001004317
• UniProt登錄號: Q6ZN17
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... LIN28B(389421)

描述

一般說明

Lin28 is a developmentally regulated RNA binding protein (RBP). It’s down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells (Viswanathan, 2008). Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (Viswanathan, 2008). The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance (Wu, 2005). Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.

特異性

Other homologies: mouse, rat 83%.

免疫原

KLH-conjugated linear peptide corresponding to the C-terminus of human Lin-28B.

應用

Anti-Lin-28B is an antibody against Lin-28B for use in WB, IH.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Lin-28B in 10 µg of human testis tissue lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Lin-28B in human testis and human liver cancer sections tissue.

品質

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Lin-28B in 10 µg of HepG2 cell lysate.

標靶描述

~35/23 kDa observed

其他說明

Concentration: Please refer to lot specific datasheet.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable