生物源
rabbit
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
mouse, human
技術
immunocytochemistry: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
rat ... Ofd1(302661)
一般說明
The cilium is a cell surface projection found in many vertebrate cells and it is required to transduce signals important for development and tissue homeostasis. OFD1 is important in cilium development, and thus it is important in cellular development and signaling. OFD1 is a component of the centrioles and appears to control the length of the centrioles in mother-daughter cells. OFD1 also plays an important role in the WNT signaling pathway and in the formation of the left-right axis in embryos. OFD1 is expressed in the cytoplasm during development in most tissues but can be found in the nucleus as well, and it is known to interact with proteins important in chromatin remodeling as well. At least one X-linked genetic mutation is associated with defects in the OFD1 gene. It is a form of oroffaciodigital syndrome characterized by abnormalities in the oral cavity, face, digits and other disfigurements that is lethal in males.
免疫原
Epitope: C-terminus
Linear peptide corresponding to the C-terminus of mouse OFD1.
應用
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-OFD1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of OFD1.
Western Blotting Analysis: A representative lot detected OFD1 in untreated and autophagy-induced/serum-starved mouse MEFs (Tang, Z., et al. (2013). Nature. 502:254-257).
Western Blotting Analysis: A representative lot detected OFD1 in wild-type female mouse embryo lysates (Ferrante, M.I., et al. (2006). Nat Genet. 38(1):112-117).
Western Blotting Analysis: A representative lot detected OFD1 in male E10.5 knock-out mouse embryo lysates (Ferrante, M.I., et al. (2006). Nat Genet. 38(1):112-117).
Immunocytochemistry Analysis: A representative lot detected OFD1 in PCM1 knockdown U2OS cells & untreated and serum-starved/autophagy-induced U2OS cells where OFD1 partially colocalizes with LC3 (Tang, Z., et al. (2013). Nature. 502:254-257).
Western Blotting Analysis: A representative lot detected OFD1 in wild-type female mouse embryo lysates (Ferrante, M.I., et al. (2006). Nat Genet. 38(1):112-117).
Western Blotting Analysis: A representative lot detected OFD1 in male E10.5 knock-out mouse embryo lysates (Ferrante, M.I., et al. (2006). Nat Genet. 38(1):112-117).
Immunocytochemistry Analysis: A representative lot detected OFD1 in PCM1 knockdown U2OS cells & untreated and serum-starved/autophagy-induced U2OS cells where OFD1 partially colocalizes with LC3 (Tang, Z., et al. (2013). Nature. 502:254-257).
品質
Evaluated by Western Blotting in U2OS cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected OFD1 in 10 µg of U2OS cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected OFD1 in 10 µg of U2OS cell lysate.
標靶描述
~120 kDa observed. Uncharacterized bands may be observed in some lysates at 45 kDa, 33 kDa & 28 kDa
外觀
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Noémie Gaudin et al.
eLife, 11 (2022-03-24)
Centrioles are formed by microtubule triplets in a ninefold symmetric arrangement. In flagellated protists and animal multiciliated cells, accessory structures tethered to specific triplets render the centrioles rotationally asymmetric, a property that is key to cytoskeletal and cellular organization in
Emanuela Senatore et al.
The EMBO journal, 40(10), e106503-e106503 (2021-05-03)
The primary cilium is a microtubule-based sensory organelle that dynamically links signalling pathways to cell differentiation, growth, and development. Genetic defects of primary cilia are responsible for genetic disorders known as ciliopathies. Orofacial digital type I syndrome (OFDI) is an
Hideki Izumi et al.
Scientific reports, 9(1), 2236-2236 (2019-02-21)
CD133 is a transmembranous protein that mainly localises to the plasma membrane in haematopoietic and neural stem cells as well as cancer stem cells. Although CD133 also localises to the cytoplasm, the mechanism of action and function of cytoplasmic CD133
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