AB15500

Anti-Calcium Transporter 1 Antibody

Chemicon^®, from rabbit

• 同義詞: CaT-1, ECAC2
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 無性繁殖: polyclonal
• application: ELISA; WB
• 物種活性: rat
• 技術: ELISA: suitable; western blot: suitable

屬性

• 生物源: rabbit
• 品質等級: 300
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: rat
• 製造商／商標名: Chemicon^®
• 技術: ELISA: suitable; western blot: suitable
• NCBI登錄號: NM_018646.2
• UniProt登錄號: Q9H1D0
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: rat ... Slc7A1(25648)

描述

一般說明

CaT-1 (rat/mouse 727 aa; human 725 aa, chromosome 7q33-q34; ~85 kDa) is also known as ECAC2, CAT-like (CATL) or TRPV6 (transient receptor potential cation channel, subfamily V, member 6) contains a cytoplasmic N-terminus, 6 ankyrin repeat domains, 6 transmembrane domains, an extracellular pore region between TM5-6, and cytoplasmic C-terminus. CaT-1 shows 75% sequence identity to epithelial Ca++ channel (ECaC) protein from rabbit kidney. In rat, CaT-1 transcript (3-Kb) is expressed in duodenum, proximal jejunum cecum and colon but not in kidney; whereas ECaC in rabbit is expressed in apical cell membranes in distal part of nephron (kidney), duodenum, jejunum and placenta.

特異性

Calcium transporter 1 (CaT-1, ECAC2). The immunogen sequence has no significant homology with CaT-2 or other proteins.

Other species have not yet been tested. The immunogen sequence is 100% conserved in mouse, 88% in humans and 94% in Cat-1/htrp8A and htrp8b proteins.

免疫原

Synthetic peptide near the C-terminus of rat CaT-1.

應用

Anti-Calcium Transporter 1 Antibody detects level of Calcium Transporter 1 & has been published & validated for use in ELISA & WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blot: 1-10 µg/mL using ECL. The expected size of CaT-1 is ~83 kDa. However, posttranslational modification may alter its size depending upon the species and cell type.
Immunohistochemistry: Not tested. It is recommended that the antibody be tried at 2-20 µg/mL
ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide/well.
Optimal working dilutions must be determined by end user.

外觀

ImmunoAffinity Purified

Liquid in PBS with 0.1% BSA.

儲存和穩定性

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids