AB1548

Anti-NMDAR2A&B Antibody, pan

Chemicon^®, from rabbit

• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: mouse, guinea pig, rat, human, rabbit, avian
• 製造商／商標名: Chemicon^®
• 技術: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI登錄號: NM_000833.2
• UniProt登錄號: Q12879; Q13224
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... GRIN2A(2903)

描述

特異性

Western blot analysis of transfected cells shows the antibody recognizes both NR2A and NR2B subunits equally. There may be a very slight recognition of NR2C and NR2D. Western blot analysis of rat brain shows a single band comigrating with NMDAR2A and NMDAR2B expressed in transfected cells. AB1548 has also been used effectively in Western blot of the membrane fraction of rabbit cortex and avian brain homogenates. No cross-reaction is seen with NMDAR1 or other glutamate receptor subunits.

免疫原

Synthetic peptide (LNSCSNRRVYKKMPSIESDV) corresponding to the C-terminus of rat NMDAR2A receptor subunit conjugated to BSA.

應用

Anti-NMDAR2A&B Antibody, pan detects level of NMDAR2A&B & has been published & validated for use in IH(P), IC, IH, IP & WB.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Western blot analysis can be done at final concentrations of 0.25-0.5 μg/mL; recognizes a single band in membrane preparations of rat hippocampal neurons ~170,000-180,000 under reduced, SDS PAGE. AB1548 can be used for immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations recommended are 0.5-1.0 μg/mL.

The antibody can be used for immunopreciptation of detergent solubilized receptor from brain or transfected cells. For most cases 5 μg of antibody is used with immobilized Protein A.

Optimal working dilutions must be determined by end user.

外觀

Affinity purified using the immunogen peptide. Lyophilized from PBS containing 1% BSA.



Note: lot 0509010940 was shipped in liquid format; Do NOT add additional water to this lot of material.

儲存和穩定性

Maintain lyophilized material at 2-8°C or -20°C. Reconstitute to 500 μL with sterile distilled water. Store reconstituted material frozen (-20°C or -80°C) in undiluted aliquots for up to six months. Avoid repeated thawing and refreezing. Diluted antibody containing 0.1% sodium azide for Western blot analysis can be stored for several weeks and reused repeatedly.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids