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70920

Sigma-Aldrich

Rosetta(DE3)pLacI Competent Cells - Novagen

Escherichia coli, rod shaped

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About This Item

分類程式碼代碼:
41106202
NACRES:
NA.81
生物源:
Escherichia coli
增長模式:
adherent or suspension
形態學:
rod shaped
製造商/商標名:
Novagen®

產品名稱

Rosetta(DE3)pLacI Competent Cells - Novagen, Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.

生物源

Escherichia coli

品質等級

製造商/商標名

Novagen®

儲存條件

OK to freeze

增長模式

adherent or suspension

形態學

rod shaped

技術

microbiological culture: suitable

運輸包裝

dry ice

儲存溫度

−70°C

一般說明

Genotype: FompT hsdSB (rB mB) gal dcm (DE3) pLacIRARE (CamR)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Expression host strains for pETBlue and pTriEx vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7lac promoter in the absence of inducer. The NovaBlue host strain is recommended for initial cloning with pETBlue and pTriEx vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA.

Note that these strains are not recommended for use with pET, pACYCDuet, pCDF, pRSF, pCOLADuet or pETcoco vectors.

.Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters. In Rosetta(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.

成分

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlRosetta(DE3)pLacI Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

法律資訊

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

分析證明 (COA)

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