407143-M

Anti-IP₃ Receptor Rabbit pAb

liquid, Calbiochem^®

• 分類程式碼代碼: 12352203

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: liquid
• 包含: ≤0.1% sodium azide as preservative
• 物種活性: human, canine, mouse, rat, porcine, bovine
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 同型: IgG
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ITPR1(3708) ; mouse ... Itpr1(16438) ; rat ... Itpr1(25262)

描述

一般說明

Rabbit polyclonal antibody purified by ammonium sulfate precipitation and ion-exchange (DEAE) column chromatography. Recognizes the ~260 kDa IP₃ receptor protein.

Recognizes all of the ~260 kDa IP₃ receptor subtypes.

This Anti-IP₃ Receptor Rabbit pAb is validated for use in ELISA, FC, Immunoblotting, IF, IHC, IP, Radioimmunoassay for the detection of IP₃ Receptor.

免疫原

a synthetic peptide corresponding to amino acids in the C-terminal cytoplasmic domain of the IP₃ receptor

應用

ELISA (1:2000)

Flow Cytometry (1:1000)

Immunoblotting (1:250)

Immunofluorescence (1:200)

Immunohistochemistry (1:200)

Immunoprecipitation (1:200)

Radioimmunoassay (1:10000)

警告

Toxicity: Standard Handling (A)

外觀

In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.

重構

Following initial thaw, aliquot and freeze (-20°C).

其他說明

Bourguignon, L.Y.W., et al. 1994. J. Cell Physiol. 159, 29.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.
Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int.17, 751.

Variables associated with assay conditions will dictate the proper working dilution.



Recommended Protocol for Immunoblotting using anti-IP₃ Receptor Antibodies



NOTE: For protein loading, we recommend 1 x 10⁶ cells/gel lane or 100 μg/gel lane.



• TBST

• 20 mM Tris-HCl, pH 7.6

• 150 mM NaCl

• 0.05% Tween^®-20 detergent



1. After transfer, block in 15 ml of TBST with 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody).

2. Dilute primary antibody in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST.

3. Incubate with polyclonal antibody for 1 h or with monoclonal antibody for 2 h.

4. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.

5. Dilute secondary antibody also in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST, followed by HRP.

6. Shake for 1 h.

7. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.

8. Mix development solution (1:1) (Amersham ECL kit).

9. In between two pieces of transparency add development solution. Allow to sit for approximately 1 min before exposure to film.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable