375230

HGF, Mouse, Recombinant

HGF, Mouse, Recombinant Recombinant, consists of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.

• 同義詞: Hepatopoietin A
• 分類程式碼代碼: 12352203

屬性

• 品質等級: 100
• 化驗: ≥95% (as seen by a 60 kDa (α chain) and a 33 kDa (β chain) bands under reducing conditions., SDS-PAGE)
• 形狀: solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze
• 雜質: <1.0 EU/μg protein Endotoxin
• 運輸包裝: ambient
• 儲存溫度: −20°C

描述

一般說明

Recombinant, mouse hepatocyte growth factor (HGF) consisting of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.

生化／生理作用

Cell Proliferation:

ED₅₀ = 10-30 ng/ml as measured in a cell proliferation assay using IMCD-3 mouse epithelial cells.



Functional ELISA

K_D<0.2 nM as measured by binding to C-Met (HGF Receptor).

警告

Toxicity: Standard Handling (A)

重構

Reconstitute to 10 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

其他說明

Corso, S., et al. 2005. Trends Mol. Med.11, 284.
Karihaloo, A., et al. 2005. Nephron Exp. Nephrol.100, e40.
Urbanek, K., et al. 2005. Circ. Res.97, 663.
Hammond, D.E., et al. 2004. Curr. Top. Microbiol. Immunol.286, 21.
Rosario, M. and W. Birchmeier. 2004. Dev. Cell7, 3.
Gheradi, E., et al. 2003. Proc. Natl. Acad. Sci.100, 12039.
Chiu, S.J., et al. 2002. J. Biomed. Sci.9, 261.
Tanaka, Y., et al. 2002. Biochem. Biophys. Res. Commun.299, 472.
Maeshima, A., et al. 2000. Kid. Int.58, 1511.
Lesk, A.M. and W.D. Fordham. 1996. J. Mol. Biol.258, 501.
Saelman, E.U.M., et al. 1995. J. Cell Sci.108, 3531.
Mineo, R., et al. 1994. Endocrinology145, 4355.
Mizuno, K., et al. 1994. J. Biol. Chem.269, 1131.
Sasaki, M., et al. 1994. Biochem. Biophys. Res. Commun.199, 772.
Montesano, R., et al. 1991. Cell67, 901.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3