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219461

Sigma-Aldrich

组织蛋白酶K,His•Tag®,人,重组,大肠埃希菌

Cathepsin K, His•Tag, Human, Recombinant, E. coli, CAS 94716-09-3, of the papain superfamily of cysteine proteinases plays a role in osteoclast-mediated bone resorption and collagen degradation.

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About This Item

CAS號碼:
酶委員會編號:
分類程式碼代碼:
12352202
NACRES:
NA.77

重組細胞

expressed in E. coli

品質等級

化驗

≥95% (SDS-PAGE)

形狀

liquid

比活性

≥900 mU/mg protein

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

運輸包裝

wet ice

儲存溫度

−70°C

一般說明

注意:1 mU = 1百万单元。
组织蛋白酶K是半胱氨酸蛋白酶木瓜蛋白酶超家族的成员,在破骨细胞介导的骨吸收和胶原降解中起重要作用。它主要由破骨细胞表达。最近报道也在成纤维细胞中表达。活性异常会导致骨质减少性疾病,例如骨质疏松症症和致密性成骨不全症症。包含C-端His•Tag。
组织蛋白酶K是半胱氨酸蛋白酶木瓜蛋白酶超家族的成员,在破骨细胞介导的骨吸收和胶原降解中起重要作用。它主要由破骨细胞表达,最近的研究也表明它在成纤维细胞中表达。组织蛋白酶K活性异常,无论是太高还是太低,都会导致骨质减少性疾病,例如骨质疏松症症和致密性成骨不全症症。该重组酶包含C端His•Tag序列。

包裝

请参考特定浓度批号的标签。

警告

毒性:标准处理(A)

單位定義

一个单位定义为在37°C,pH 5.5下,每分钟水解0.4 µmol Z-FR-AMC的酶量。

外觀

在50 mM NaOAC,pH 5.5中。通过添加10 mM MMTS停止激活,可以在还原条件下将其去除。

重構

初次解冻后,分装冻存(-70°C)。

其他說明

Delaisse, J.M., et al. 2003.Microsc.Res. Tech.61, 504.
Dodds, R.A.2003.Cell Biochem.Funct.21, 231.
Everts, V., et al. 2003.Calcif.Tissue Int.73, 380.
Hou, W.S., et al. 2003.Biol. Chem.384, 891.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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