14-766-M

Tie2 (Y1108F) Protein, active, 10 µg

• 分類程式碼代碼: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

屬性

• 生物源: human
• 品質等級: 300
• 分子量: M_w 42 kDa
• 製造商／商標名: Upstate^®
• 技術: activity assay: suitable (kinase)
• NCBI登錄號: NM_000459
• UniProt登錄號: Q02763; Q02858 (mouse)

描述

一般說明

Expressed by baculovirus in Sf21 insect cell.

N-terminal 6His-tagged recombinant human Tie2 residues 771-end.

Tyrosine 1108 has been identified as an autophosphorylation site in human Tie2. The Y1106F substitution in murine Tie2 (analogous to Y1108F in human Tie2) has been shown to block cell migration by disrupting binding of Tie2 to the docking protein Dok-R (Jones N., et al., Mol Cell Biol.(2003);23:2658-2668 and Murray B.W., et al., Biochemistry.(2001);40:10243-10253).

應用

Active, N-terminal 6His-tagged recombinant human Tie2 residues 771-end. For use in Kinase Assays.

Research Category
Inflammation & Immunology

生化／生理作用

• Protein Target: Tie2
• Target Sub-Family:TK

包裝

Also available in 250μg size-call for pricing and availability and reference catalog number 14-766M when ordering the 250μg size.

品質

routinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1)

外觀

50mM Tris/HCl pH8.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Format: Ni²⁺/NTA-agarose

儲存和穩定性

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately s

其他說明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

安全資訊

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H317
• 防範說明: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危險分類: Skin Sens. 1
• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable