推薦產品
等級
RIA grade
化驗
≥98% (agarose gel electrophoresis)
形狀
solid
製造商/商標名
Calbiochem®
儲存條件
OK to freeze
雜質
≤3.0 ash
≤5.0% moisture
顏色
off-white
溶解度
0.15 M sodium chloride: 10 mg/mL
正離子痕跡
heavy metals: ≤10 ppm
運輸包裝
ambient
儲存溫度
2-8°C
一般說明
牛血清白蛋白(BSA)是一种α-螺旋状的非糖基化球状蛋白,含有17个二硫键。
牛血清白蛋白,组分V(RIA和ELISA级)是可用作稳定剂和载体蛋白的纯化血浆蛋白。在非变性条件下通过改良Cohn方法和辛酸热激处理制备。在150 mM NaCl中10%的pH值为6.8-7.4。
應用
牛血清白蛋白,组分V(RIA和ELISA级)已用于:
- 在免疫组化(IHC)分析中用于胎盘组织切片的封闭
- 作为Krebs缓冲液组分,用于体外葡萄糖刺激胰岛素分泌(GSIS)实验和药物/棕榈酸制备
- 作为溶剂对照孵育小鼠全血,用于研究体内血小板聚集
生化/生理作用
牛血清白蛋白(BSA)是细胞培养基的重要成分,可运输脂肪酸、激素和药物。它是酶联免疫吸附试验(ELISA)中常用的封闭剂。
警告
毒性:标准处理(A)
重構
在复溶后,分装并冷冻保存(-20°C)。储备溶液在-20°C下可稳定保存至多6个月。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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