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Merck
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文件

126593

Sigma-Aldrich

白蛋白、牛血清、组分V、RIA和ELISA级

同義詞:

白蛋白、牛血清、组分V、RIA和ELISA级

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About This Item

CAS號碼:
分類程式碼代碼:
12352202
NACRES:
NA.25

等級

RIA grade

化驗

≥98% (agarose gel electrophoresis)

形狀

solid

製造商/商標名

Calbiochem®

儲存條件

OK to freeze

雜質

≤3.0 ash
≤5.0% moisture

顏色

off-white

溶解度

0.15 M sodium chloride: 10 mg/mL

正離子痕跡

heavy metals: ≤10 ppm

運輸包裝

ambient

儲存溫度

2-8°C

一般說明

牛血清白蛋白(BSA)是一种α-螺旋状的非糖基化球状蛋白,含有17个二硫键。
牛血清白蛋白,组分V(RIA和ELISA级)是可用作稳定剂和载体蛋白的纯化血浆蛋白。在非变性条件下通过改良Cohn方法和辛酸热激处理制备。在150 mM NaCl中10%的pH值为6.8-7.4。

應用

牛血清白蛋白,组分V(RIA和ELISA级)已用于:
  • 在免疫组化(IHC)分析中用于胎盘组织切片的封闭
  • 作为Krebs缓冲液组分,用于体外葡萄糖刺激胰岛素分泌(GSIS)实验和药物/棕榈酸制备
  • 作为溶剂对照孵育小鼠全血,用于研究体内血小板聚集

生化/生理作用

牛血清白蛋白(BSA)是细胞培养基的重要成分,可运输脂肪酸、激素和药物。它是酶联免疫吸附试验(ELISA)中常用的封闭剂。

警告

毒性:标准处理(A)

重構

在复溶后,分装并冷冻保存(-20°C)。储备溶液在-20°C下可稳定保存至多6个月。

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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