07-890-I

Anti-phospho PTEN (Ser385) Antibody

from rabbit, purified by affinity chromatography

• 同義詞: Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, phospho PTEN (Ser385), Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 物種活性（以同源性預測）: mouse (based on 100% sequence homology)
• 技術: inhibition assay: suitable (peptide); western blot: suitable
• NCBI登錄號: NP_000305
• UniProt登錄號: P60484
• 運輸包裝: wet ice
• 目標翻譯後修改: phosphorylation (pSer385)
• 基因資訊: human ... PTEN(5728) , TEP1(7011)

描述

一般說明

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (EC 3.1.3.16, EC 3.1.3.48, EC 3.1.3.67; UniProt P60484; also known as Mitochondrial PTENalpha, Phosphatase and tensin-like protein, Mutated in multiple advanced cancers 1, Mitochondrial phosphatase and tensin protein alpha, MMAC1 phosphatase and tensin homolog deleted on chromosome 10, Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN) is encoded by the PTEN (also known as 10q23del, BZS, CWS1, DEC, GLM2, TEP1, MMAC1, MHAM, PTEN1) gene (Gene ID 5728) in human. PTEN protein catalyzes the removal of phosphate from phosphoserine, phosphothreonine, and phosphotyrosine residues on substrate proteins, as well as the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate. PTEN plays a major role in controlling cell growth and proliferation, and PTEN gene mutains are found in a large number of cancers at high frequency. PTEN activity, stability, localization, and conformation are controlled through complex regulatory mechanisms, including the reversible phosphorylations of several Ser/Thr (S/T) residues in its unfolded C-terminal tail region (PTEN-Ctail, residues 351–403). PTEN S370, S380, T382, T383 and S385 are known target sites by casein kinase II (CK2), while S361, T363, S362, and T366 are phosphorylated by glycogen synthase kinase-3 β (GSK3β). In addition, Polo-like kinase 1 (Plk1), Plk3, the microtubule associated serine/threonine (MAST) kinase family, the serine/threonine kinase 11 (STK11, LKB1), and the casein kinase 1 (CK1) have also been reported to phosphorylate PTEN.

特異性

PTEN phosphorylated on Serine residue 385

免疫原

Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-PTEN (Ser385).

應用

Peptide Inhibition Analysis: Target specificity of this antibody was confirmed by antibody blocking using the immunogen peptide and the corresponding non-phosphorylated peptide prior to Western blotting detection of Ser385 phosphorylated C124S PTEN mutant in lysate from transfected MDA-MB-468 cells. (Lysate courtesy of Dr James Hastie, University of Dundee, UK).

This Anti-phospho PTEN (Ser385) Antibody is validated for use in Western Blotting and Peptide Inhibition Assay for the detection of phospho PTEN (Ser385).

品質

Evaluated by Western Blotting in MDA-MB-468 cells transfected with PTEN mutant (C124S) cell lysate.

Western Blotting Analysis: This antibody (0.2 µg/mL) detected Ser385 phosphorylation of the dual lipid and protein phosphatase dead C124S PTEN mutant in lysates of C124S PTEN-transfected MDA-MB-468 cells (Lysate courtesy of Dr James Hastie, University of Dundee, UK).

標靶描述

~54 kDa observed

其他說明

Concentration: Please refer to lot specific datasheet.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable