07-1221

Anti-PP2A-B′ (B56) Antibody

from rabbit

• 同義詞: PP2A phosphatase activator, PP2A, subunit B′, PP2A, subunit B′, PR53 isoform, Phosphotyrosyl phosphatase activator, protein phosphatase 2A activator, regulatory subunit 4, protein phosphatase 2A, regulatory subunit B′, protein phosphatase 2A, regulatory
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: rat, human, mouse, bovine
• 技術: western blot: suitable
• 同型: IgG
• NCBI登錄號: NM_006243
• UniProt登錄號: Q15257
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PTPA(5524)

描述

一般說明

PP2A is a heterotrimeric type 2 Ser/Thr phosphatase, composed of a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B′/PR61, and B′′/PR72 . The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma . The B′ (or B56) family consists of at least seven splice variants derived from five genes. RNA′s for the two members of the B′′ family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B′ subunits are conserved from yeast to mammals. PTPA is a member of the B′ family that is required for activation of PP2A by an undefined mechanism. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades.

特異性

This antibody is specific to PP2A-B’ subunits.

免疫原

Epitope: Internal

The immunogen used for the purified peptide conjugated to KLH corresponds to the sequence NH2-Val-Ser-Ser-Pro-His-Phe-Gln-Val-Ala-Glu- Arg-Ala-Leu-Tyr-COOH.

應用

Detect PP2A-B’ (B56) using this Anti-PP2A-B’ (B56) Antibody validated for use in WB.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Kinases & Phosphatases

品質

Routinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis: 1:500-1:1,500 dilution of this antibody detected PP2A-B′ (B56) in 10 μg of multiple cell lysates.

標靶描述

~56 kDa

聯結

Replaces: AB4088

外觀

Purified

Format: Purified

Purified rabbit polyclonal IgG in PBS with 0.08% sodium azide.

儲存和穩定性

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
HeLa Cell Lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable