06-1289

Anti-Cyclin T1 Antibody

serum, from sheep

• 同義詞: CDK9-associated C-type protein, Human immunodeficiency virus-1 expression, cyclin C-related protein, cyclin T1, cyclin T1b, human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1, subunit of positive elongation transcription factor b
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: sheep
• 品質等級: 100
• 抗體表格: serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human, mouse
• 技術: western blot: suitable
• 同型: IgG
• NCBI登錄號: NP_001231.2
• UniProt登錄號: O60563
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CCNT1(904) ; mouse ... Ccnt1(12455)

描述

一般說明

Cyclin T1, which is also known as CCNT, is a member of the cyclin family and functions as a CDK kinase regulator. Cyclin T1 is one of regulatory cyclins (T1, T2a, T2b, and K) that is closely associated with CDK9 kinase forming a heterodimer called P-TEFb. This kinase complex is involved in phosporylating the carboxyl-terminal domain (CTD) of the large RNA polymerase II subunit which stimulate the elongation upon initiation. The cyclin T1/CDK9 complex has also been referred to as tat-associated kinase. Tat is an HIV protein that targets the cyclin T1/CDK9 complex to activate the transcription of the virus.

特異性

Rat (85% sequence homology).

This antibody recognizes Cyclin T1.

免疫原

Epitope: Unknown

His-tagged recombinant protein corresponding to human Cyclin T1.

應用

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-Cyclin T1 Antibody (Sheep Polyclonal Antibody) validated in WB to detect Cyclin T1 also known as CDK9-associated C-type protein, cyclin C-related protein.

Western Blot Analysis: A 1:1000 dilution from a previous lot detected Cyclin T1 in 10 µg of HEK293, HeLa, HepG2, Jurkat, and PC3 cell lysates.

品質

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:1000 dilution of this antibody detected Cyclin T1 in 10 µg of HeLa cell lysate.

標靶描述

Cyclin T1 is expected to be ~85 kDa This antibody detects a ~85 kDa band in most cell lines. It also detects the degraded forms of Cyclin T1 (~70 kDa, ~60 kDa, et al), which is beneficial for IP applications. According to independent laboratory, this antibody can immunoprecipitate multiple forms of Cyclin T1.

外觀

Unpurified

Sheep polyclonal serum with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
HeLa cell lysate

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1