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Merck
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文件

05-666

Sigma-Aldrich

抗BRCA2抗体，克隆5.23

clone 5.23, Upstate®, from mouse

同義詞:

Anti BRCC2, Anti-BROVCA2, Anti-FACD, Anti-FAD, Anti-FAD1, Anti-FANCD, Anti-FANCD1, Anti-GLM3, Anti-PNCA2, Anti-XRCC11

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

100

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

5.23, monoclonal

物種活性

human

製造商／商標名

Upstate®

技術

immunocytochemistry: suitable
western blot: suitable

同型

IgG

NCBI登錄號

NM_000059

UniProt登錄號

P51587

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... BRCA2(675)

特異性

BRCA2

免疫原

GST融合蛋白，对应人BRCA2蛋白的2959-3418位残基

應用

用抗BRCA2抗体，克隆5.23（小鼠单克隆抗体）检测BRCA2，已证明可在WB、ICC中起作用。
研究子类别
细胞周期，DNA 复制&修复
研究类别
表观遗传学&核功能

品質

已通过免疫印迹法对HeLa核提取物进行常规评估

標靶描述

390kDa

外觀

0.1M Tris-甘氨酸（pH 7.4），0.15M NaCl与0.05％叠氮化钠。
形式：纯化
蛋白G层析

儲存和穩定性

储存于2-8°C。 收到后，在取下瓶盖之前，将小瓶离心并轻轻混合溶液。分装到微量离心管中，并储存于2-8°C。

分析報告

对照
阳性抗原对照：货号12-309，HeLa细胞核提取物。添加 等体积Laemmli样品还原性缓冲液至10 μL提取物并 煮沸5分钟以还原制剂。在小型凝胶每个泳道上样20 μg 经还原的提取物。

其他說明

由于许可协议限制，无法购买该产品进行转售。

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Loredana Moro et al.
The Journal of biological chemistry, 280(23), 22482-22491 (2005-04-05)
BRCA2 is a tumor suppressor gene that when mutated confers an increased susceptibility to developing breast and prostate carcinoma. Besides its role in mediating DNA repair, new evidence suggests that BRCA2 may also play a role in suppressing cancer cell
Jimmy M-M Kwok et al.
Molecular cancer research : MCR, 8(1), 24-34 (2010-01-14)
The transcription factor Forkhead box M1 (FOXM1) is a key regulator of cell proliferation and is overexpressed in many forms of primary cancers, leading to uncontrolled cell division and genomic instability. To address the role of FOXM1 in chemoresistance, we
Loredana Moro et al.
The Journal of biological chemistry, 281(31), 22100-22107 (2006-06-07)
Aberrant interaction of carcinoma cells with basement membranes (BM) is a fundamental pathophysiological process that initiates a series of events resulting in cancer cell invasion and metastasis. In this report, we describe the results of our investigations pertaining to the
Yongjun Tan et al.
Molecular and cellular biology, 27(3), 1007-1016 (2006-11-15)
The forkhead box M1 (FoxM1) transcription factor regulates expression of cell cycle genes essential for DNA replication and mitosis during organ repair and cancer progression. Here, we demonstrate that FoxM1-deficient (-/-) mouse embryonic fibroblasts and osteosarcoma U2OS cells depleted in
Miyoung Lee et al.
Nucleic acids research, 49(10), 5588-5604 (2021-05-13)
Cancer-causing missense mutations in the 3418 amino acid BRCA2 breast and ovarian cancer suppressor protein frequently affect a short (∼340 residue) segment in its carboxyl-terminal domain (DBD). Here, we identify a shared molecular mechanism underlying their pathogenicity. Pathogenic BRCA2 missense
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