913812

2-(Azidomethyl)nicotinic acid imidazolide

≥95%

• 同義詞: 2-(Azidomethyl)-3-(1H-imidazole-1-carbonyl)pyridine, NAI-N3, RNA SHAPE probe, icSHAPE reagent
• 經驗公式（希爾表示法）: C₁₀H₈N₆O
• CAS號碼: 1612756-29-2
• 分子量：: 228.21
• MDL號碼: MFCD31560475
• 分類程式碼代碼: 12352106

屬性

• 品質等級: 100
• 化驗: ≥95%
• 形狀: powder
• mp: 74-80 °C
• 儲存溫度: −20°C

描述

應用

2-(Azidomethyl)nicotinic acid imidazolide (NAI-N3) is an RNA icSHAPE probe for live-cell RNA structure profiling across the genome. icSHAPE -- or in vivo click selective 2′-hydroxyl acylation and profiling experiment -- uses NAI-N3 in a chemoaffinity method to probe RNA structure. NAI-N3 is an azido version of the cell-permeable SHAPE reagent 2-methylnicotinic acid imidazolide (NAI) that permits the tagging of NAI-N3-modified RNA with a biotin moiety for subsequent capture via streptavidin. Not only do such strategies further the understanding of RNA structure in living cells but also provide a tool for identifying regions that may be susceptible to therapeutic targeting. Recently, the azidomethylnicotinyl (AMN) group of NAI-N3 was demonstrated to block the function of gRNA and CRISPR systems, which could be reactivated by removing the AMN groups with Staudinger reduction (DPBM phosphine), overall providing a means to control nucleic acid cleavage and gene editing in live cells.

其他說明

Conditional control of RNA-guided nucleic acid cleavage and gene editing

SnapShot: RNA Structure Probing Technologies

Multiplex Aptamer Discovery through Apta-Seq and Its Application to ATP Aptamers Derived from Human-Genomic SELEX

Structural imprints in vivo decode RNA regulatory mechanisms

安全資訊

• 象形圖: GHS02,GHS07
• 訊號詞: Danger
• 危險聲明: H242,H315,H319
• 防範說明: P210 - P235 - P302 + P352 - P305 + P351 + P338 - P370 + P378 - P403
• 危險分類: Eye Irrit. 2 - Self-react. C - Skin Irrit. 2
• 儲存類別代碼: 5.2 - Organic peroxides and self-reacting hazardous materials
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable