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Key Documents

906611

Sigma-Aldrich

DLAM-MεVproS-13CH3 Methyl Labeling Kit

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.12

技術

bio NMR: suitable

品質等級

運輸包裝

dry ice

儲存溫度

−70°C

一般說明

DLAM-MεVproS-13CH3 Kit has 13CH3 isotopomer precursors and contains protocol instructions for creation of isotopically-labeled proteins.

應用

DLAM-MεVproS-13CH3 is an optimized kit to produce proteins with -13CH3 isotopomer on the methyl groups of methionine and valine residues. This kit has been tested with protein isotopic labeling in E. coli. It is used in TROSY NMR for revealing the mechanisms of dynamic molecular machines of N-terminal sequence
For detection of long-range nOes or for the NMR study of large proteins

包裝

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

象形圖

Corrosion

訊號詞

Danger

危險聲明

危險分類

Skin Corr. 1B

儲存類別代碼

8A - Combustible corrosive hazardous materials


分析證明 (COA)

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Tomasz L Religa et al.
Science (New York, N.Y.), 328(5974), 98-102 (2010-04-03)
The proteasome catalyzes the majority of protein degradation in the cell and plays an integral role in cellular homeostasis. Control over proteolysis by the 20S core-particle (CP) proteasome is achieved by gated access of substrate; thus, an understanding of the
Silke Wiesner et al.
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that

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