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重要文件

A6426

Sigma-Aldrich

消泡剂 204

mixture of organic polyether dispersions

同義詞:

organic antifoam

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About This Item

MDL號碼:
分類程式碼代碼:
12161900
NACRES:
NA.25
暫時無法取得訂價和供貨情況

生物源

synthetic

品質等級

形狀

liquid

技術

microbiological culture: suitable

黏度

400 cP(lit.)

密度

1.01 g/cm3 at 25 °C (lit.)

儲存溫度

room temp

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一般說明

消泡剂起着有效抑泡剂的作用。[1]204消泡剂含有100%活性成分,是一种有机非硅聚丙烯基聚醚分散体的混合物。它不含矿物油。204消泡剂本身可被视为一种表面活性剂,但其不含其他表面活性剂。该产品为合成产物,并非来源于动植物。204消泡剂可以反复灭菌。204消泡剂良好的流动性使得它可以根据需要泵入发酵罐。用于微生物培养基时,Sigma建议其起始浓度在0.005%到0.01%之间。

應用

消泡剂204已用于:

  • 在膜内异戊二烯定量过程中,抑制类囊体溶液鼓泡[2]
  • 用作分批补料连续培养发酵培养基组分[3][4]
  • 抑制发酵罐中的剧烈起泡

特點和優勢

  • 用于细胞生物学和生物化学研究的多功能表面活性剂
  • 适用于微生物学培养

其他說明

包含 100% 活性成分,是多种无硅酮有机消泡剂的混合物,在多元醇中分散。可反复灭菌。
如需了解生化试剂系列的更多信息,请填写此表

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

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存取文件庫

Juan J Barrero et al.
New biotechnology, 60, 85-95 (2020-10-13)
Pichia pastoris (Komagataella spp.) has become one of the most important host organisms for production of heterologous proteins of biotechnological interest, many of them extracellular. The protein secretion pathway has been recognized as a limiting process in which many roadblocks
Concentration of isoprene in artificial and thylakoid membranes
Harvey CM, et al.
Journal of Bioenergetics and Biomembranes, 47(5), 419-429 (2015)
A novel noncovalent complex of chorismate mutase and DAHP synthase from Mycobacterium tuberculosis: protein purification, crystallization and X-ray diffraction analysis
M Okvist, et al.
Acta Crystallographica Section F, Structural Biology and Crystallization Communications, 65(10), 1048-1052 (2009)
Martin Kavšček et al.
BMC systems biology, 9, 72-72 (2015-10-28)
Yarrowia lipolytica is a non-conventional yeast that is extensively investigated for its ability to excrete citrate or to accumulate large amounts of storage lipids, which is of great significance for single cell oil production. Both traits are thus of interest
Vasil D'Ambrosio et al.
Metabolic engineering, 61, 369-380 (2020-07-28)
Engineering living cells for production of chemicals, enzymes and therapeutics can burden cells due to use of limited native co-factor availability and/or expression burdens, totalling a fitness deficit compared to parental cells encoded through long evolutionary trajectories to maximise fitness.

Questions

1–7 of 7 Questions  
  1. What is the molecular weight of this product? Mean and dispersion? We are trying to assess passage through membranes.

    1 answer
    1. The molecular weight is not determined for this product. An internal note suggests the antifoam's molecular weight is approximately 3,000 daltons, but this is not verified.

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. May i ask if you have the toxicological safety data (such as LD50) of Antifoam 204 (Cat # A6426)?

    1 answer
    1. The toxicological data can be found in Section 11 of the Safety Data Sheet. This document may be accessed by entering the product number in the SDS section at the link below: https://www.sigmaaldrich.com/documents-search?tab=sds

      Helpful?

  5. I am using Antifoam 204 in vaccine fermentation process as an antifoaming agent. Regulatory expectation is to varify residual content of 204 in final product. Do you have any suggestion on which analytical method should one explore for such a characteriza

    1 answer
    1. This product is intended for research use only. It is not suitable for human or veterinary use. Unfortunately, a method for residual detection is not available. However, a brief search of the literature reveals that several methods may be deployed, including LC/MS/MS and LS/CAD. Please see the links below to review publications regarding testing for residual amounts of antifoam.
      https://onlinelibrary.wiley.com/doi/pdf/10.1002/j.2050-0416.1977.tb06809.x
      https://www.eurofinsus.com/media/447588/9207_residual_impurities_bioprocessing.pdf
      https://www.bioprocessintl.com/process-monitoring-and-controls/analytical-strategies-for-monitoring-residual-impurities-encountered-in-bioprocessing

      Residual level tests for antifoam may require a dedicated piece of equipment due to the antifoam properties.
      Various general recommendations may be of interest:
      a. Use an antifoam sensor/ probe or pump to measure the amount of antifoam in solution
      b. NMR
      c. Viscosity

      Helpful?

  6. Quelle est la molécule du antifoam 204? Est ce un polyethylene glycol classique?

    1 answer
    1. Antifoam 204 contains 100% active components and is a mixture of organic non-silicone polypropylene-based polyether dispersions. It does not contain mineral oil. The exact composition is considered proprietary by the supplier.

      Helpful?

  7. May I ask if you have the LD50 information of Antifoam 204 (Cat#A6426)?

    1 answer
    1. Unfortunately, there is no LD50 information available for this product. A brief search of the literature revealed a publication that reports an LD50 of > 5000 mg/kg in rats. Please see the link below to review this article:
      https://www.sciencedirect.com/science/article/abs/pii/B9781774670088500092

      Helpful?

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