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G005805

GC Analysis of Pesticides in Avocado on SLB®-5ms (20 m x 0.18 mm I.D., 0.36 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep+, Fast GC Analysis

application for SPE, suitable for GC

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About This Item

technique(s)

gas chromatography (GC): suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: Dispersive (QuEChERS)
sample/matrix: Avocado spiked with pesticides at 20 ng/g
extraction tube: Supel QuE Acetate extraction tube (55234-U)
extraction process: add 25 mL of acetonitrile; shake for 1 minute; add contents of Supel QuE acetate extraction tube; shake immediately for 1 minute; centrifuge at 3200 rpm for 5 minutes
clean-up tube: Supel Que Z-Sep+ cleanup tube (55296-U)
clean-up process: transfer 3 mL of the acetonitrile layer into a Supel Que Z-Sep+ cleanup tube; shake for 1 minute; centrifuge at 3400 rpm for 3 minutes; draw off supernatant for LVI-GCMS analysis
column: SLB-5ms, 20 m x 0.18 mm I.D., 0.36 μm (28576-U)
oven: 70 °C (2 min), 15 °C/min to 325 °C (6 min)
inj. temp.: 60 °C (0.28 min), 600 °C/min to 325 °C (5 min)
detector: MSD
MSD interface: 330 °C
carrier gas: helium, 1 mL/min, constant
injection: PTV solvent vent; 10 μL, injection dispense speed 15000 μL/min
liner: 4 mm I.D. FocusLiner with taper
sample: pesticides spiked at 20 ng/g

suitability

application for SPE

application(s)

agriculture
food and beverages

Analysis Note

About this analysis: This application demonstrates the use of dispersive SPE for the extraction of pesticides from Avocado. Cleanup is achieved using Z-Sep + for fat removal. FInal analysis of the extracts was done by LVI-GC/MS SIM on an SLB-5ms column.

Legal Information

SLB is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    Trifluralin
    α-1,2,3,4,5,6-Hexachlorocyclohexane
    Hexachlorobenzene
    Dimethoate
    Simazine
    Pentachloronitrobenzene
    Lindane
    Heptachlor
    Malathion
    Metolachlor
    4,4′-DDT
    Methoxychlor
    Coumaphos
    Deltamethrin
    See All (14)

SPE tube or plate

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