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G005697

HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si (25 cm Column)

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)
mobile phase: [A] hexane; [B] ethyl acetate
gradient: 10 to 30% B in 10 min; held at 30% B for 2 min
flow rate: 1.0 mL/min
column temp.: 30 °C
detector: UV, 290 nm
injection: 10 μL

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

Ascentis silica is used in the analysis of fat-soluble vitamins using a normal phase gradient. These include vitamin K 3 (menadione), vitamin E (alpha and gamma tocopherol), and vitamin D3 (cholecalciferol).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    (±)-α-Tocopherol, analytical standard
    Menadione (K3), analytical standard
    (+)-γ-Tocopherol, analytical standard
    Cholecalciferol (D3), analytical standard

analytical column

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Description
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standard

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