823483
TSKgel® Protein A-5PW (20 µm) HPLC Columns
L x I.D. 3.5 cm × 4.6 mm, Protein A phase
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About This Item
UNSPSC Code:
41115709
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product name
TSKgel® Protein A-5PW HPLC Column, 20 μm particle size, L × I.D. 3.5 cm × 4.6 mm
material
PEEK
product line
TSKgel®
manufacturer/tradename
TSKgel®
parameter
20 bar pressure (290 psi)
4-30 °C temperature
technique(s)
HPLC: suitable
L × I.D.
3.5 cm × 4.6 mm
matrix
polymeric particle platform
matrix active group
Protein A phase
particle size
20 μm
separation technique
affinity
storage temp.
2-8°C
General description
TSKgel Protein A-5PW is a 20 μm, 4.6 mm ID × 3.5 cm column for high performance affinity chromatography. Made of PEEK hardware, this column has been designed for the rapid separation and robust quantification of a
variety of antibodies. Monoclonal antibodies from harvested cell culture media can be captured and accurately quantitated in less than 2 minutes per injection.
The TSKgel Protein A-5PW column can be used for more than 2,000 injections without regeneration or cleaning. Packed with hydroxylated methacrylic polymer beads, this column is designed with a high degree
of crosslinking, which allows a high flow rate for chromatography while still maintaining chromatographic efficiency, peak width and resolution. The recombinant protein A ligand is a code-modified hexamer of the C domain.
An enhanced rProtein A ligand is bound to the TSKgel 5PW base bead via multipoint attachment resulting in excellent base stability in 0.1 mol/L NaOH.
variety of antibodies. Monoclonal antibodies from harvested cell culture media can be captured and accurately quantitated in less than 2 minutes per injection.
The TSKgel Protein A-5PW column can be used for more than 2,000 injections without regeneration or cleaning. Packed with hydroxylated methacrylic polymer beads, this column is designed with a high degree
of crosslinking, which allows a high flow rate for chromatography while still maintaining chromatographic efficiency, peak width and resolution. The recombinant protein A ligand is a code-modified hexamer of the C domain.
An enhanced rProtein A ligand is bound to the TSKgel 5PW base bead via multipoint attachment resulting in excellent base stability in 0.1 mol/L NaOH.
Footnote
Highlights
- Wide dynamic range with high sample concentration load for mAb quantitation
- Fast analysis: 1-2 min/analysis
- High sensitivity for mAb determination
- Long lifetime: .2,000 injections per column with no sign of deterioration
Legal Information
TSKgel is a registered trademark of Tosoh Corporation
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