ZMS1223

Anti-CD1d Antibody, clone 51.1 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Antigen-presenting glycoprotein CD1d, R3G1
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 51.1, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 37.72 kDa
• purified by: using protein G
• species reactivity: human
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable
• isotype: IgG2bκ
• epitope sequence: Extracellular domain
• Protein ID accession no.: NP_001306074.1
• UniProt accession no.: P15813
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... CD1D(912)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 51.1 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects CD1d. It targets an epitope within the extracellular domain.

Immunogen

Fc-fusion protein of Human CD1d, expressed in Murine NSO cells.

Application

Quality Control Testing

Evaluated by Flow Cytometry in Jurkat cells.

Flow Cytometry Analysis: 1 µg of this antibody detected CD1d in one million Jurkat cells.

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD1d in Jurkat cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Antigen-presenting glycoprotein CD1d (UniProt: P15813; also known as R3G1, CD1d) is encoded by the CD1D gene (Gene ID: 912) in human. Cd1d is a single-pass type 1 membrane glycoprotein, which is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 20-301), a transmembrane domain (aa 302-322), and a short cytoplasmic domain (aa 323-335). It is expressed at high levels by human cortical thymocytes, and its expression is also observed on certain T cell leukemias. It serves as a key antigen-presenting molecule involved in the selection and activation of NK cells. It is a major-histocompatibility-complex (MHC) class-I-like molecule that presents self-lipid and non-self-lipid antigens to invariant natural killer T (iNKT) cells. Following engagement of the invariant T cell receptor (iTCR) by CD1d lipid complexes, iNKT cells proliferate, produce cytokines, and become cytotoxic, regulating innate and adaptive immune responses. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Oleinika, K., et al. (2018). Nat. Commun. 9(1); Article 684; Spada, FM., et al. (2000). Eur. J. Immunol. 30; 3468-3477; Exley, M., et al. (2000). Immunology. 100(1): 37-47).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable