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Z0276

Sigma-Aldrich

Anti-phospho-ZAP-70 (pTyr315/pTyr319) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

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About This Item

MDL number:
MFCD07370447
UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

human, mouse

technique(s)

western blot: 1:1,000 using Jurkat cells treated with H2O2.

UniProt accession no.

P43403

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... ZAP70(7535)
mouse ... Zap70(22637)

General description

ZAP-70 is a protein tyrosine kinase that is expressed in natural killer cells and T-lymphocytes. ZAP-70 interacts with the ζ chain of the T cell antigen receptor (TCR) and subsequently modulates cell signaling. Phosphorylation of Tyr315 and Tyr319 residues regulates TCR-mediated signal transduction . Anti-phospho-ZAP-70 pTyr315/pTyr319) antibody is specific for human ZAP-70. The product is expected to react with mouse ZAP-70 (as its 100% homologous to human ZAP-70), but has not been tested for it.

Immunogen

synthetic phosphopeptide derived from the region of human ZAP-70 that contains tyrosines 315 and 319.

Application

Anti-phospho-ZAP-70 pTyr315/pTyr319) antibody is suitable for use in western blot (1:1,000 using Jurkat cells treated with H2O2 and immunoblot.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg2+and Ca2+), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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B L Williams et al.
The EMBO journal, 18(7), 1832-1844 (1999-04-15)
Accumulating evidence indicates that the interdomain B regions of ZAP-70 and Syk play pivotal roles in the coupling of T-cell antigen receptor (TCR) stimulation to the activation of downstream signaling pathways. The interdomain B region of ZAP-70 contains at least
V Di Bartolo et al.
The Journal of biological chemistry, 274(10), 6285-6294 (1999-02-26)
Following T cell antigen receptor (TCR) engagement, the protein tyrosine kinase (PTK) ZAP-70 is rapidly phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family PTK Lck. These events have been implicated in
A C Chan et al.
Cell, 71(4), 649-662 (1992-11-13)
Protein-tyrosine kinases (PTKs) play an integral role in T cell activation. Stimulation of the T cell antigen receptor (TCR) results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR zeta chain, which can mediate

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