W4387
Wee1, active from rat
N-terminal GST-tagged, solution
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About This Item
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biological source
rat
Quality Level
recombinant
expressed in E. coli
form
solution
mol wt
~110 kDa
UniProt accession no.
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Gene Information
rat ... Wee1(308937)
Application
Wee1, active from rat may be used to study apoptotic signaling.
Biochem/physiol Actions
Wee1 is a protein tyrosine kinase that phosphorylates Cdc2 on Tyr15 and hence inactivates the CDK1 and CDK2, which facilitates in the negative regulation of entry of cells into mitosis.[1] Increased level of wee1 stimulates Human Immunodeficiency Virus type 1 (HIV-1) Vpr induced cell cycle arrest at G2/M transition followed by apoptosis.[2]
Protein inhibitor of Cdk2 kinase
Unit Definition
One unit of Wee 1 is defined as the amount of Wee 1 which inhibits 90% of the activity of 10 units of Cdk2
Physical form
Solution in 50 mM Tris-HCl, pH 7.5, 1 mM EDTA, 1 mM DTT, 10% Glycerol
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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P Russell et al.
Cell, 49(4), 559-567 (1987-05-22)
Fission yeast wee1- mutants initiate mitosis at half the cell size of wild type. The wee1+ activity is required to prevent lethal premature mitosis in cells that overproduce the mitotic inducer cdc25+. This lethal phenotype was used to clone wee1+
F S Goes et al.
European journal of biochemistry, 268(8), 2281-2289 (2001-04-12)
The Wee1 protein kinase negatively regulates entry into mitosis by mediating the inhibitory tyrosine phosphorylation of Cdc2-cyclin B kinase. The stability and activity of Wee1 from the fission yeast Schizosaccharomyces pombe is critically dependent on functional Hsp90 chaperones. Here we
Huidong Yuan et al.
Journal of virology, 78(15), 8183-8190 (2004-07-16)
Human immunodeficiency virus type 1 (HIV-1) Vpr induces cell cycle arrest at the G(2)/M transition and subsequently apoptosis. Here we examined the potential involvement of Wee-1 in Vpr-induced G(2) arrest. Wee-1 is a cellular protein kinase that inhibits Cdc2 activity
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