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TOX1
In Vitro Toxicology Assay Kit, MTT based
Synonym(s):
mitochondrial activity assay
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.84
Pricing and availability is not currently available.
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usage
1 kit sufficient for 1,000 tests
Quality Level
packaging
pkg of 1 kit
storage condition
dry at room temperature
λmax
570 nm
application(s)
cell analysis
detection
detection method
colorimetric
storage temp.
2-8°C
General description
This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results. The key component is (3- [4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) or MTT. Solutions of MTT, dissolved in medium or balanced salt solutions without phenol red, are yellowish in color. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple formazan crystals which are insoluble in aqueous solutions. Absorbance of converted dye is measured at a wavelength of 570nm.
Application
In Vitro Toxicology Assay Kit, MTT based has been used to perform an (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay to measure the cytotoxicity of gold nanoparticles (AuNP).[1] It has also been used to examine cell viability[2] in SH-SY5Y cultures in response to 6-hydroxydopamine (6-OHDA) treatment.[3]
Biochem/physiol Actions
Conversion of MTT to a water-insoluble colored formazan derivative which is then solubilized in acidic isopropanol.
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Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1 - STOT SE 3
Target Organs
Central nervous system, Respiratory system
Storage Class Code
3 - Flammable liquids
Flash Point(F)
53.6 °F
Flash Point(C)
12 °C
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Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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What is the Department of Transportation shipping information for this product?
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Is the MTT assay quantitative?
1 answer-
The MTT asay is a method to assess cell viability. This assay is a semiquantitative assay. The assay is used to compare the viability changes in treated cells to untreated cells. The absorbance is indicative of the cell number. The higher the absorbance, the greater the number of viable cells present. Most researchers compare the absorbance of the two samples as a ratio (ABS treated cells/ABS untreated cells) to get a fold increase/decrease in cell number.
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What is the difference between product CGD1, Cell Growth Determination Kit and product TOX1, In Vitro Toxicology Assay Kit?
1 answer-
Product CGD1 and TOX1 are both MTT based assays. The major difference between product CGD1 and TOX1 is that TOX1 contains 10 % Triton X-100 in the solubilization solution for better lysis of the cells and solubilization of the MTT. If there is complete cell lysis, the results obtained with both kits would be the same. The MTT in this kit is a solution, whereas the TOX1 assay kit contains the MTT as a powder that is solubilized before use.
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