P8248

Monoclonal Anti-Profilin antibody produced in mouse

~2 mg/mL, clone 2B8 (MAbPRF45a), purified immunoglobulin, buffered aqueous solution

• MDL number: MFCD01865559
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2B8 (MAbPRF45a), monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~14 kDa
• species reactivity: Arabidopsis thaliana
• concentration: ~2 mg/mL
• technique(s): immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 1-2 μg/mL using total extracts of Arabidopsis flower
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: Arabidopsis thaliana ... PRF4(829055)

Description

General description

Profilins are highly conserved pan-allergens that occur in plant foods, pollen and latex. Profilins have five distinct isoforms (PRF1-5) that bind with actin filaments to regulate cytoskeletal organization. PRF4 and PRF5 are cytoplasmic proteins that are expressed in mature pollens . Monoclonal Anti-Profilin antibody is specific for Arabidopsis pollen profilin (PRF4 and PRF5). The antibody detects tobacco and maize profilin (approx. 14 kDa), but does not react with constitutive specific profilins (PRF1, 2, and 3).

Immunogen

profilin PRF4 protein.

Application

Monoclonal Anti-Profilin antibody is suitable for use in western blot (1-2 μg/mL using total extracts of Arabidopsis flower), indirect ELISA and immunohistochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves