slides can be dried by leaving them at room temperature overnight or by heating them at 37-40 degrees C for several hours. However, it is standard lab policy to wait at least 3 or 4 days before filing the slides, especially if excess mounting media was used. Storing slides in a cabinet before they are fully dry could lead to them sticking together.
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greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
Quality Level
sustainability
Greener Alternative Product
color
clear
density
0.840 g/cm3
application(s)
hematology
histology
greener alternative category
storage temp.
room temp
Application
Organo/Limonene Mount™ is a ready-to-use mounting medium is made with limonene, a natural product from orange peels. It is good for coverslipping stained tissues and cell smears that can be dehydrated with organic solvents.[1][2][3] This mounting medium is suitable for organic solvent resistant immunohistochemistry (IHC) chromogens as well as an excellent choice for mounting H and E stained slides.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to toluene based mounting mediums.
Linkage
Organo/Limonene Mount™ is comparable to Clarion™ mounting medium.
Legal Information
Clarion is a trademark of Biomeda Corp.
Organo/Limonene Mount is a trademark of ImmunoBioScience Corp.
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Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Asp. Tox. 1 - Flam. Liq. 3 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
Flash Point(F)
118.4 °F
Flash Point(C)
48 °C
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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How long does it take for slides to dry when coverslipped with Organo/Limonene O8015?
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Is the organo/limonene mounting media a hard-setting media, or does it require extra sealing on the coverslip (i.e. nail polish)?
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This product is a hard-setting mounting medium, sealing is not required. Simply add 2-3 drops of mounting medium and carefully apply the coverslip, avoiding air bubbles. Slides may be dried overnight at room temperature or by incubating at 37-40°C for several hours.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Why is Organo/Limonene Mount (Product No. O8015) preferable to a mounting media that is xylene based?
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There are more health hazards associated with xylene in comparison to products derived from natural products, such as Organo/Limonene Mount.
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The instructions for use of Product No. O8015, Organo/Limonene Mount™, call for using xylene for clearing. Can limonene or other xylene substitutes be considered acceptable for use?
1 answer-
Yes, limonene would be considered a suitable xylene substitute. There are however a wide variety of xylene substitutes available on the market. No exhaustive comparative studies have been performed using Organo/Limonene Mount with each and every available xylene substitute.
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Organo/Limonene Mount (Product No. O8015) is stated to be suitable for use with chromogens for immunohistochemistry. Does this?
1 answer-
Suitable chromogens for immunohistochemistry would be DAB, or DAB with an added metal such as nickel or cobalt. Alkaline phosphatase chromogens would also be suitable for use with Oregano/Limonene Mount.
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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What are the instructions on preparing a sample for use with Product No. O8015 (Organo/Limonene Mount™)?
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Dehydrate slides with dehydrating agents, such as graded ethanol or reagent alcohol. Dehydration refers to removing the water by using a series of grades alcohols. Typically slides spend 1-5 minutes each in a series of alcohols. Sequences vary by laboratory. A typical sequence would be 1 station of 80% alcohol, 2-3 changes of 95% alcohol, 2-3 changes of absolute alcohol. The slides are then processed through 2-3 changes of xylene. After finishing with the last xylene solution, the slides are ready for coverslipping with the Organo/Limonene Mount.
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