MDQ1
Imprint® Methylated DNA Quantification Kit
To measure global DNA methylation shifts from as low as 10 ng DNA
Synonym(s):
DNA Methylation Quantification, DNA methylation shift
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
41116158
NACRES:
NA.54
Recommended Products
General description
Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.
Application
Imprint® Methylated DNA Quantification Kit has been used in global DNA methylation analysis.
Features and Benefits
- ELISA based format
- Procedure completes in 4 easy to follow steps
- All reagents supplied including control methylated DNA
- Detection limit is 5 ng of fully methylated DNA
- Input DNA may be as low as 10-200 ng
- Procedure completes in 3.5 hours
- 96 well format allows option of studying single samples or high-throughput studies
- Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
- May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Kit Components Also Available Separately
Product No.
Description
SDS
- M8570Methylated Control DNA, 50 ng/μLSDS
related product
Product No.
Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Rafael Guerrero-Preston et al.
Epigenetics, 5(6), 539-546 (2010-06-05)
Environmental exposures in-utero may alter the epigenome, thus impacting chromosomal stability and gene expression. We hypothesized that in utero exposures to maternal smoking and perfluoroalkyl compounds (PFCs) are associated with global DNA hypomethylation in umbilical cord serum. Our objective was
Magdalena Gryzinska et al.
Biochemical genetics, 51(7-8), 554-563 (2013-04-05)
DNA methylation is an epigenetic modification that plays an important role in the normal development and function of organisms. The level of DNA methylation is species-, tissue-, and organelle-specific, and the methylation pattern is determined during embryogenesis. DNA methylation has
Resistance training and redox homeostasis: Correlation with age-associated genomic changes
Dimauro I, et al.
Redox Biology, 10, 34-44 (2016)
Kazuhisa Nakano et al.
Journal of immunology (Baltimore, Md. : 1950), 190(3), 1297-1303 (2013-01-02)
Rheumatoid arthritis (RA) is a chronic inflammatory disease in which fibroblast-like synoviocytes (FLS) exhibit an aggressive phenotype. Although the mechanisms responsible are not well defined, epigenetic determinants such as DNA methylation might contribute. DNA methyltransferases (DNMTs) are critical enzymes that
Felicitas Thol et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 29(21), 2889-2896 (2011-06-15)
To study the incidence and prognostic impact of mutations in DNA methyltransferase 3A (DNMT3A) in patients with acute myeloid leukemia. A total of 489 patients with AML were examined for mutations in DNMT3A by direct sequencing. The prognostic impact of
Articles
Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.
Protocols
Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service