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MAK017

Sigma-Aldrich

Lactose Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

cancer; neonatal diseases; pediatric diseases

storage temp.

−20°C

General description

Lactose is a dissacharide formed by the condensation of one galactose and one glucose molecule. Lactose is the major sugar in the milk of most species, typically present between 2-8%. The enzyme lactase hydrolyzes lactose to its constituent monosaccharides. In neonates, glucose released via the action of lactase is a major energy source.

Application

Lactose Assay Kit has been used to measure lactose content in milk.[1]

Suitability

Suitable for the direct measurement of lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.)

Principle

In this assay, lactose concentration is determined by an enzymatic assay, in which lactose is converted to galactose and glucose. The galactose is subsequently oxidized, resulting in a colorimetric (570 nm)/ fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the lactose initially present. This kit is suitable for use with various biological samples and has a linear range of detection between 0.2-1.0 nmole lactose for the fluorometric assay and 2-10 nmoles lactose for the colorimetric assay.

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8L29K06240
8E26K06240
7M20K06240
7L04K06240
7J20K06240

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Effect of high-fat diet on secreted milk transcriptome in midlactation mice.
Chen Y, et al.
Physiological Genomics, 49(12), 747-762 (2017)
Yulie E Meneses et al.
Journal of dairy science, 99(5), 3396-3407 (2016-03-01)
Water scarcity is threatening food security and business growth in the United States. In the dairy sector, most of the water is used in cleaning applications; therefore, any attempt to support water conservation in these processes will have a considerable
Y Chen et al.
Physiological genomics, 49(12), 747-762 (2017-11-03)
High-fat diet (HFD) during lactation alters milk composition and is associated with development of metabolic diseases in the offspring. We hypothesized that HFD affects milk microRNA (miRNA) and mRNA content, which potentially impact offspring development. Our objective was to determine
Holly DeRosa et al.
eNeuro, 9(4) (2022-08-23)
Breastfeeding confers robust benefits to offspring development in terms of growth, immunity, and neurophysiology. Similarly, improving environmental complexity, i.e., environmental enrichment (EE), contributes developmental advantages to both humans and laboratory animal models. However, the impact of environmental context on maternal
Adrienne A Cheng et al.
American journal of physiology. Endocrinology and metabolism, 320(3), E438-E452 (2021-01-12)
Obesity is a prevailing problem across the globe. Women who are obese have difficulty initiating and sustaining lactation. However, the impact of genetics and diet on breastfeeding outcomes is understudied. Here we explore the effect of diet and genotype on

Questions

1–3 of 3 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. what is the substrate in the kit ? Is it lactose? can I have a look at the manual before ordering it ?

    1 answer

    1. The mechanism employed in this kit is the enzymatic conversion of the disaccharide lactose to the component monosaccharides glucose and galactose. The galactose component is then oxidized, resulting in a colorimetric/fluorimetric response.

      Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/324/674/mak017bul.pdf

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