L6274
Laminin from human placenta
liquid, BioReagent, suitable for cell culture
Synonym(s):
Cell culture grade laminin, Human Laminin
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
Recommended Products
biological source
human placenta
Quality Level
product line
BioReagent
form
liquid
concentration
0.5 mg/mL in Tris-buffered saline
technique(s)
cell culture | mammalian: suitable
surface coverage
1‑2 μg/cm2
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... LAMB1(3912)
Application
Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Biochem/physiol Actions
Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.
Components
Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product is purified from human placenta using pepsin treatment and immunoaffinity chromatography. SDS-PAGE results in two major bands at 50 kDa and 130-160 kDa.
Caution
It is recommended to store this product at -70°C, where it will remain stable for two years.
Preparation Note
This product is supplied at a concentration of 0.5 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is 0.2 μm filtered. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a sterile balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Satu Marja Myllymäki et al.
PloS one, 6(5), e19453-e19453 (2011-05-17)
Formation of apical compartments underlies the morphogenesis of most epithelial organs during development. The extracellular matrix (ECM), particularly the basement membrane (BM), plays an important role in orienting the apico-basal polarity and thereby the positioning of apical lumens. Integrins have
Chiyi Xiong et al.
Scientific reports, 10(1), 520-520 (2020-01-18)
The tyrosine kinase receptor EphB4 is frequently overexpressed in ovarian and other solid tumors and is involved in interactions between tumor cells and the tumor microenvironment, contributing to metastasis. Trans-interaction between EphB4 and its membrane-bound ligand ephrin B2 (EFNB2) mediates
Sanne L N Brouns et al.
Scientific reports, 10(1), 11910-11910 (2020-07-19)
In haemostasis and thrombosis, platelet, coagulation and anticoagulation pathways act together to produce fibrin-containing thrombi. We developed a microspot-based technique, in which we assessed platelet adhesion, platelet activation, thrombus structure and fibrin clot formation in real time using flowing whole
Pere Roca-Cusachs et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(38), 16245-16250 (2009-10-07)
A key molecular link between cells and the extracellular matrix is the binding between fibronectin and integrins alpha(5)beta(1) and alpha(v)beta(3). However, the roles of these different integrins in establishing adhesion remain unclear. We tested the adhesion strength of fibronectin-integrin-cytoskeleton linkages
Elizabeth K Davis et al.
Neural development, 3, 32-32 (2008-11-07)
Wnt proteins comprise a large class of signaling molecules that regulate a variety of developmental processes, including synapse formation. Previous studies have shown Wnts to be involved in both the induction and prevention of synapses in a number of different
Articles
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
Protocols
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service