Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

KSPQ12012

KiCqStart® SYBR® Green Primers

Predesigned primers for gene expression analysis

Synonym(s):

Pre-designed RT-qPCR Primers, SYBR Green RT-qPCR Primers

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

storage temp.

−20°C

General description

Overview
Compatible with any thermal cycler, KiCqStart Primers are ready-to-order, pre-designed primer pairs for quantifying gene expression by SYBR Green I RT-qPCR (Reverse Transcription Quantitative Real-Time PCR). To comply with MIQE, they have been developed using sophisticated bioinformatics tools and validated in silico to avoid off-target amplification1. KiCqStart Primers are available as up to three ranked sets of forward and reverse primer pairs for all genes from common model organisms.

1SNP loci have been avoided during the design process when possible. Please consult dbSNP to determine if your gene has unavoidable SNPs.

To learn more about KiCqStart Primers visit our Frequently Asked Questions section.

Specifications
Design characteristics
  • GC Content: 20 - 80%2
  • Melting Temperature: 55.0 ± 5 ºC
  • Secondary Structure: None
  • Excessive 3′ Clamping: Minimal
  • Homology: None

Oligo characteristics
  • Molecule: DNA (all sequences verified by mass spec QC); no modifications
  • Contents: 1 forward and 1 reverse primer
  • Length: 18 - 24 bases2
  • Purification: RP cartridge
  • Format: 3 OD minimum yield, dry; 1 oligonucleotide per tube

Sequences are provided at the time of shipment.

295% of primer pairs fall within these specifications

Rank
Each gene has up to three alternative primer pairs, which are identified as follows:
  • 1: The middle of one primer overlaps an exon splice site
  • 2: The 5′ or 3′ end of one primer overlaps an exon splice site
  • 3: The amplicon, but neither primer, overlaps an exon splice site
  • 4: Primer overlap is not possible for various reasons, e.g. the gene has only one exon (primer pairs with this rank may lead to co-amplification of genomic DNA).

Application

Product use: gene expression analysis

Features and Benefits

KiCqStart Primers are sensitive, specific, and will save you time and money by eliminating the tedious steps required to develop an effective SYBR Green I RT-qPCR Assay.
Benefits include:
  • Target analysis is done
  • Design characteristics are set
  • Oligo characteristics are set
  • Easy to order

Physical properties

Properties include:
  • Available species: human, mouse, rat, zebrafish, cow, horse, Arabidopsis, chicken, dog, rabbit, pig, cat, rice, soybean, rhesus, Drosophila, C. elegans, corn, X. laevis, Guinea Pig, and Chinese Hamster.
  • Components: two primers, one oligo per tube
  • Shipping condition: dry, ambient temperature
  • Storage condition: dry or wet single-use aliquots

Other Notes

Frequently Asked Questions section is available for this product.

Legal Information

KiCqStart is a registered trademark of QIAGEN Beverly Inc.
SYBR is a registered trademark of Life Technologies

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Mara Ribeiro Almeida et al.
Molecular nutrition & food research, 60(7), 1615-1624 (2016-03-05)
Vitamin B6 plays crucial roles on brain development and its maternal deficiency impacts the gamma-aminobutyric acid (GABA)ergic, serotonergic, glutamatergic, and dopaminergic systems in offspring. However, the molecular mechanisms underlying these neurological changes are not well understood. Thus, we aimed at
Agata Nyga et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 107(5), 1352-1362 (2018-09-28)
We aimed to find a biomarker for patients with adverse reaction to metal debris (ARMD) due to a metal-on-metal (MoM) hip implant. First, we compared molecular markers of hypoxia-inducible factor (HIF) pathway activation (BNIP3, GLUT1, HO1, VEGF, and HIF1A) and
Aaminah Khan et al.
Nature communications, 12(1), 971-971 (2021-02-14)
Diffuse intrinsic pontine glioma (DIPG) is an incurable malignant childhood brain tumor, with no active systemic therapies and a 5-year survival of less than 1%. Polyamines are small organic polycations that are essential for DNA replication, translation and cell proliferation.
Ana Paula Oliveira De Quadros et al.
Journal of toxicology and environmental health. Part A, 83(13-14), 495-508 (2020-06-23)
Sm. (Rosaceae) is a plant traditionally used in Brazil and some other countries to treat diarrhea, stomach diseases, and as an analgesic, antimicrobial, antihypertensive, and as well as other pharmacological properties. The aim of this study was to examine cytotoxic
Sun Woong Kim et al.
The ocular surface, 16(4), 463-469 (2018-07-11)
To evaluate the role of PPARγ in regulating meibocyte differentiation and lipid synthesis in a human meibomian gland epithelial cell line (hMGEC). HMGEC were exposed to the PPARγ agonist, Rosiglitazone, from 10-50 μM. Cultures were also exposed to specific PPARγ antagonist
View All Related Papers

Articles

KiCqStart® SYBR® Green Primers FAQs

Primer pairs detect prevalent eukaryotic genes, but may co-amplify other transcripts, emphasizing the importance of comprehensive primer design.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service