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ligand
recombinant protein L (E. coli), mammalian free
packaging
pack of 5 mL
manufacturer/tradename
Cytiva 17-5478-06
storage condition
(20% Ehtanol)
matrix
rigid, highly cross-linked agarose
avg. part. size
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
working range
2-10(pH interval where the medium can be operated without significant change in function.)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
General description
Capto™ L combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains.
The high binding capacity and affinity to a broad range of antibody fragments makes this resin an excellent capture platform for antibody fragments containing kappa light chains. Common uses include domain antibody (dAb), scFv, and Fab purification.
Capto™ L resin requires minimal optimization and produces high purity in one separation step, resulting in significant savings during process development. This resin is also advantageous in manufacturing, because high selectivity of affinity capture reduces the number of subsequent polishing steps.
Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, such as PreDictor plates, PreDictor RoboColumn, and HiScreen™ prepacked columns.
Features and Benefits
- Suitable for the capture of a wide range of antibody fragments such as Fabs, dAbs, and single-chain fragment variable (scFv) .
- High binding capacity, low ligand leakage, and selectivity for a broad range of antibody fragments reduces process time and amount of resin.
- Enables a platform approach to antibody fragment purification.
- Excellent productivity, process economy, and process design flexibility due to rigid matrix.
- Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
- Fulfills industrial demands for security of supply, robust performance, and regulatory support.
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Protocols
This page shows how to purify antibody fragments by affinity chromatography using Capto L products from Cytiva.
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