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G4423

Sigma-Aldrich

β-Glucanase from Trichoderma longibrachiatum

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Synonym(s):

endo-1,3(4)-β-glucanase, endoglucanase

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About This Item

Enzyme Commission number:
3.2.1.6 (BRENDA, IUBMB)
MDL number:
MFCD00131488
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

form

powder

Quality Level

200

specific activity

≥1.0 units/mg solid

composition

protein, ≥10%

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

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greener alternative category

, Enabling

General description

β -Glucanase represents a group of carbohydrate enzymes which break down glycosidic bonds within beta-glucan.[1] It forms the main constituent of fungal cell walls and could be a potential structural and storage polysaccharide of marine macro-algae. It has the ability to degrade fungal cell walls and may be involved in defense mechanism of plants against pathogenic fungi.[1]
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles.

Application

β -Glucanase was used as a cellulase enzyme in the combined biological and chemical pretreatment method for lignocellulosic ethanol production from energy cane. It was also used in the enzymatic saccharification of cellulose and production of ethanol. [2]

Biochem/physiol Actions

A mixture composed mainly of β-1→3 / 1→4-glucanase, xylanase, and cellulase activities. β-glucosidase, β-xylosidase, α-L-arabinofuranosidase, amylase, and protease activities are also present.[3][4]

Unit Definition

One unit will liberate 1.0 μmole of glucose from cellulose in one hr at pH 5.0 at 37 °C (2 hr incubation time).

Physical form

contains maltodextrin, silica and sodium benzoate

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000338179
0000335829
0000335829
0000338179
0000269972

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David J Adams
Microbiology (Reading, England), 150(Pt 7), 2029-2035 (2004-07-17)
The fungal cell wall is a complex structure composed of chitin, glucans and other polymers, and there is evidence of extensive cross-linking between these components. The wall structure is highly dynamic, changing constantly during cell division, growth and morphogenesis. Hydrolytic
Combined biological and chemical pretreatment method for lignocellulosic ethanol production from energy cane;
Sara Shields, Raj Boopathy
Renewable Bioresources null
Ravindra Bansal et al.
Current genetics, 65(4), 953-964 (2019-03-07)
Trichoderma spp. are widely used as commercial biofungicides, and most commercial formulations are conidia based. Identification of genes that regulate conidiation would thus be of help in genetic reprogramming of these species to optimize sporulation. In this study, we constructed
Marie-Therese Eckstein et al.
Current biology : CB, 30(23), 4799-4807 (2020-10-10)
The human gut microbiota is composed of diverse microbes that not only compete but also rely on one another for resources and access to microhabitats in the intestine [1, 2]. Indeed, recent efforts to map the microbial biogeography of the
Chengwei Hua et al.
Applied microbiology and biotechnology, 88(2), 509-518 (2010-07-21)
In this study, a novel beta-1,3-1,4-glucanase gene (designated as PtLic16A) from Paecilomyces thermophila was cloned and sequenced. PtLic16A has an open reading frame of 945 bp, encoding 314 amino acids. The deduced amino acid sequence shares the highest identity (61%)
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Questions

1–3 of 3 Questions  

  1. How much protease activity is present?

    1 answer

    1. The minimum activity of this ?-glucanase is 1 unit per milligram. The activity varies from lot to lot and is reported in the Certificate of Analysis. This product has not been tested for any additional protease activity. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/g4423#product-documentation

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  2. Is this product suitable for use in food preparation?

    1 answer

    1. No. Unless otherwise stated, this product is intended for research use only. It is not suitable for any other purpose, which includes, but is not limited to, unauthorized commercial use, in vitro diagnostic use, or therapeutic use in humans or animals.

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  3. Hi, I am planning to use β-Glucanase G4423 to digest the Pyhtium inosidieus cell wall to let the probes for In situ hybridozation . May I know the conditions, concentration and dilution buffer for this product? Thanks Best

    1 answer

    1. This product has not been testd in In Situ Hybridization in house. Below is a link to an article citing this product in ISH.

      https://www.nature.com/articles/s42003-021-02693-y

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