Skip to Content
Merck
All Photos(3)

Key Documents

View All Documentation

G2500

Sigma-Aldrich

Gelatin from porcine skin

gel strength 300, Type A

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

CAS Number:
9000-70-8
EC Number:
232-554-6
MDL number:
MFCD00081638
UNSPSC Code:
12352202
NACRES:
NA.61
Pricing and availability is not currently available.

biological source

Porcine skin

Quality Level

200

type

Type A

Assay

70-90% (biuret)

form

powder

technique(s)

cell culture | mammalian: suitable

gel strength

~300 g Bloom

solubility

H2O: soluble 50 mg/mL

Looking for similar products? Visit Product Comparison Guide

General description

Gelatin is a hydrocolloid. Bovine or porcine bone and hide are its commercial sources.[1] It is a collagen derivative and comprises protein as a major component with minerals and water.[2]

Application

Gelatin from porcine skin has been used:
  • for coating glass-bottomed plates for culturing A375 cells and in gelatin degradation assay[1]
  • for coating glass slides in the histological studies with brain sections[3]
  • in the synthesis of gelatin methacrylate (GelMA) prepolymer for encapsulating cells on to 3D printed hydrogels[4]
  • to prepare thiol-functionalized gelatin (GelSH) hydrogel for 3D projection bioprinting[5]

Gelatin from porcine skin is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Biochem/physiol Actions

Gelatin polymer is an alternative biomaterial to collagen for cell and tissue culture.[2] It is a much sorted gelling agent useful as a thickener and food ingredient. Gelatin is also used as a foaming and binding agent and as well as in the pharmaceutical industry.[1] It is biodegradable and biocompatible with biomedical applications especially in biomaterials for cardiac and vascular tissue regeneration.[2]

Components

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gell strength of 300 and is soluble in water at 50 mg/mL.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000410030
0000410029
0000410028
0000410028
0000410030

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Gabriele Pitingolo et al.
Micromachines, 10(4) (2019-04-24)
Traditional two-dimensional (2D) cell culture models are limited in their ability to reproduce human structures and functions. On the contrary, three-dimensional (3D) microtissues have the potential to permit the development of new cell-based assays as advanced in vitro models to
Sami Zaqout et al.
Frontiers in neuroanatomy, 10, 38-38 (2016-04-12)
Golgi staining remains a key method to study neuronal morphology in vivo. Since most protocols delineating modifications of the original staining method lack details on critical steps, establishing this method in a laboratory can be time-consuming and frustrating. Here, we
Céline Bidoret et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(33), 14126-14131 (2009-08-12)
The detailed characterization of synaptic plasticity has led to the replacement of simple Hebbian rules by more complex rules depending on the order of presynaptic and postsynaptic action potentials. Here, we describe a mechanism endowing a plasticity rule with additional
Nicola Contessi Negrini et al.
Acta biomaterialia, 87, 61-75 (2019-01-18)
When adipose tissue (AT) is impaired by trauma or disease, AT engineering could provide a shelf-ready structural and functional restoration as alternative to current clinical treatments, which mainly aim at aesthetic replacement. Yet, the lack of an efficient vascular network
J P Gobetti et al.
Journal of the American Dental Association (1939), 113(2), 291-292 (1986-08-01)
The study showed that washing a gloved hand removed significant amounts of bacteria. If the proper soap or scrub is used, the gloved hand will be free of bacteria. It is suggested that all dental personnel wear gloves to protect
View All Related Papers

Articles

Photo-Crosslinkable Gelatin Hydrogel: Versatile Materials for (High Resolution) Additive Manufacturing

Discussion of synthetic modifications to gelatin, improving the three-dimensional (3D) print resolution, and resulting material properties.

Papain, Cysteine Protease, Properties & Products

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

Questions

1–3 of 3 Questions  

  1. What is the molecular weight of this Gelatin?

    1 answer

    1. The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
      Bloom Number Average Molecular Mass (Da)
      50−125 (Low Bloom) 20,000−25,000
      175−225 (Medium Bloom) 40,000−50,000
      225−325 (High Bloom) 50,000−100,000

      Please see the link below to review the general datasheet for Gelatin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdf

      Helpful?

  2. what is the amino group content of the product Gelatine from porcine skin G2500?

    1 answer

    1. The amino group content of Gelatin is not determined in-house.

      Helpful?

  3. What's the molecular weight of this gelatin

    1 answer

    1. The average molecular weight is between 50,000−100,000 Da.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service