The refractive index is 1.364 ± 0.002. This number applies to this mounting medium in solution. Refractive indexes change when the mounting media dries on the slide.
Pricing and availability is not currently available.
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Product Name
Fluoroshield™, histology mounting medium
Quality Level
form
liquid
technique(s)
immunofluorescence: suitable
pH range
7.8-8.2
solubility
water: soluble
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
General description
Fluoroshield is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula not only enables prolonged fluorescence, but also prevents rapid photobleaching (fading) of fluorochromes including FITC, Texas Red®, AMCA, Cy®, Cy3™, Cy5, Alexa Fluor™ 488, Alexa Fluor™ 594, Green fluorescent protein (GFP), tetramethylrhodamine, R-phycoerythrin (R-PE), phycocyanin (PC), and allophycocyanin (APC).
Application
Fluoroshield™ has been used:
- as a mounting medium for cultured megakaryocytes and immunostained platelets for confocal microscopy studies[1]
- as a mounting medium for 4′,6-diamidino-2-phenylindole (DAPI) stained heart sections for immunofluorescence studies[2]
- to preserve fluorescence of C6 cell smears[3]
Linkage
Fluoroshield is an aqueous-based mounting medium comparable to Gel Mount™.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Cy is a registered trademark of Cytiva
Cy3 is a trademark of Cytiva
FluoroShield is a trademark of ImmunoBioScience Corp.
Gel Mount is a trademark of Biomeda Corp.
Texas Red is a registered trademark of Life Technologies
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
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What is the refractive index of this product (Fluoroshield Histology Mounting Medium, F6182)?
1 answer-
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Does this product harden as it dries? Also, what is it's shelf life?
1 answer-
This is an aqueous mounting medium and will dry over time if exposed to air, however this is unrelated to the intended use of the product. The mounting medium is applied over the sample prior to the addition of the coverslip. See the brief protocol below:
1. Bring the vial to room temperature.
2. Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, then touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface away from light.
3. Turn the vial upside down and remove any air bubbles.
4. Apply 3-4 drops directly of mounting medium on top of the specimen and spread out evenly by tilting the slide back and forth or spread evenly with a 0.2 ml plastic pipette tip, making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide on paper towel.
5. Let stand at room temperature for about 5 minutes.
6. Apply cover slip, carefully avoiding air bubbles.
7. The specimen is ready for visualization under microscope.
8. One can seal the edges of cover slip with nail polish or any organic medium. If coverslip is not used air bubbles will appear in few days.
9. For long term storage it is recommended that the slide be stored in the dark at 2-8°C.This product is assigned an expiration date of approximately one year. Please view the sample Certificate of Analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/F6182-20ML-PWMKCT0475.pdf.
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How do I store Fluoroshield (Product Nos. F6182, F5932, F6057) ?
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This product is stored at 2-8°C. Protect from light, and do not freeze.
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How do you remove the coverslip when using Fluoroshield (Product Nos. F6182, F5932, F6057)?
1 answer-
The coverslip can be removed before sealing the edges. Soak slide in warm (37 °C) water for a few minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What fluorochromes can be used with Fluoroshield (Product Nos. F6182, F5932, F6057)?
1 answer-
This unique formula prevents rapid photobleaching of FITC, Texas Red®, AMCA, Cy®2, Cy®3, Cy®5, Alexa Fluor® 488, Alexa Fluor® 594, green fluorescent protein (GFP), tetramethylrhodamine, R-phycoerythrin (R-PE), phycocyanin (PC), and allophycocyanin (APC). Fluorescence is retained during prolonged storage at 4°C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of the Cy dyes, RP-E, PC and APC.
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Does Fluoroshield (Product Nos. F6182, F5932, F6057) contain an antifade reagent?
1 answer-
This unique formula prevents rapid photobleaching. The exact nature of the antifade is considered proprietary.
Helpful?
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How do I use Fluoroshield (Product Nos. F6182, F5932, F6057)?
1 answer-
1. Bring the vial to room temperature. 2. Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, then touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface away from light. 3. Turn the vial upside down and remove any air bubbles. 4. Apply 3-4 drops directly of mounting medium on top of the specimen and spread out evenly by tilting the slide back and forth or spread evenly with a 0.2 ml plastic pipette tip, making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide on paper towel. 5. Let stand at room temperature for about 5 minutes. 6. Apply cover slip, carefully avoiding air bubbles. 7. The specimen is ready for visualization under microscope. 8. One can seal the edges of cover slip with nail polish or any organic medium. If coverslip is not used air bubbles will appear in few days. 9. For long term storage it is recommended that the slide be stored in the dark at 2-8°C.
Helpful?
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