F6008
Ficin from fig tree latex
lyophilized powder
Synonym(s):
Debricin, Ficain, higueroxyl delabarre
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About This Item
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biological source
fig tree (latex)
form
lyophilized powder
specific activity
≥1.0 units/mg protein
mol wt
23.8 kDa
composition
Protein, 80-100% biuret
storage temp.
−20°C
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General description
Extinction Coefficient: E1% = 21.0 (280 nm)
pI: 9.0
pI: 9.0
Application
Ficin from fig tree latex has been used:
- in the proteolysis of tendon collagen
- in the digestion of Chlorophyta Ulva rigida
- to investigate it effect on protease-activated receptors 2 and 4 activation to induce itch in skin
Biochem/physiol Actions
Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)2 fragments from mouse IgG1.
Ficin is effective in disrupting the staphylococcal biofilms making it accessible antibiotics exposure.
Unit Definition
One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37°C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path).
Physical form
Lyophilized powder containing L-cysteine and trace EDTA
Preparation Note
This enzyme is soluble in water (10 mg/ml), yielding a clear solution.
inhibitor
Product No.
Description
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substrate
Product No.
Description
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Protocols
This procedure may be used for all Ficin products.
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