indirect immunofluorescence: 5-10 μg/mL using human HepG2 or rat NRK cells western blot (chemiluminescent): 0.5-1.0 μg/mL using whole cell extract of mouse NIH3T3 cells
ERGIC (ER-Golgi intermediate compartment) is related to type I membrane marker protein ERGIC-53 and rat homolog is referred as (p58) . ERGIC is a dynamic membrane system made up of tubulo vesicular clusters near to ER exit site which facilitates transport of protein from ER to Golgi. Anti-ERGIC-53/ (p58) antibody can be used as a primary antibody (diluted 1: 125) in fluorescence microscopy. It may also be used for western blotting. Rabbit anti-ERGIC-53/ (p58) antibodies react specifically with ERGIC-53/ (p58) of rat, mouse and human.
Immunogen
synthetic peptide corresponding to amino acids 158-170 of rat p58 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse and human.
Application
Anti-ERGIC-53/ (p58) antibody can be used in immunoblotting and immunofluorescence.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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