D9692
Plant Protoplast Digest/Wash Solution
Rapid isolation of viable protoplasts from plant tissue
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About This Item
UNSPSC Code:
41105500
NACRES:
NA.56
Pricing and availability is not currently available.
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General description
The Plant Protoplast Digest/Wash Solution is formulated to facilitate the rapid isolation of viable protoplasts from plant tissue. Plant cells are surrounded by a rigid, semi-permeable cell wall composed primarily of three classes of polysaccharides: cellulose, hemicellulose, and pectin.[1] The Plant Protoplast Digest/Wash Solution can be used for the digestion of the cell wall after the addition of enzymes that hydrolyze these polysaccharides, such as cellulase, pectinase, or pectolyase. The Plant Protoplast Digest/Wash Solution can be subsequently used to wash away any remaining hydrolytic enzymes after digestion is complete.
Plant protoplasts are typically used for any of a number of downstream applications. These applications include, but are not limited to, transient gene expression,[2] viral transfection assays,[3] somatic hybridization,[4] electrophysiological studies,[5] and morphological studies.[6]
Plant protoplasts are typically used for any of a number of downstream applications. These applications include, but are not limited to, transient gene expression,[2] viral transfection assays,[3] somatic hybridization,[4] electrophysiological studies,[5] and morphological studies.[6]
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Although transient gene expression using reporters such as green fluorescent protein is a versatile tool for examining gene functions and intracellular protein trafficking, the establishment of a highly efficient gene manipulation method remains a challenge in many plant species. A
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