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91060554, Reactivity: Anti-CD45RA, ovine

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About This Item

UNSPSC Code:
41106514
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biological source

Mouse x Mouse Hybridoma

ImmunoDon

BALB/c mouse spleen

Myeloma

Sp2/0

Reactivity

Anti-CD45RA, ovine

technique(s)

cell culture | mammalian: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse Sp2/0 x Mouse BALB/c spleen

Cell Line Description

The antibody is directed against the p220 component of the sheep CD45 molecule (CD45RA), expressed on all B cells and a sub-population of naive T cells.

Immunogen

Sheep lymphocytes

Application

Useful for distinguishing naive and memory T cells, FACS

Culture Medium

EMEM(EBSS) + 2mM glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

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