L132 (HeLa derivative)
89111004, human cervix, Epithelial
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About This Item
UNSPSC Code:
41106514
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product name
L132 (HeLa derivative), 89111004
biological source
human cervix
growth mode
Adherent
karyotype
2n = 46
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human cervix carcinoma
Cell Line Description
Originally derived from a human embryonic lung. Used for primary isolation of enteroviruses and also found to be sensitive to hepatitis virus. The line shows HeLa markers. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.
Application
Isolation of enteroviruses
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. The cells grow in clumps.
Other Notes
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Certificates of Analysis (COA)
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