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C9210

Sigma-Aldrich

Cyanogen bromide-activated Agarose

lyophilized powder

Synonym(s):

Agarose, cyclic carbonimidate, CNBr-Activated Agarose

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About This Item

CAS Number:
68987-32-6
MDL number:
MFCD00163385
UNSPSC Code:
41106500
NACRES:
NA.56
Pricing and availability is not currently available.

form

lyophilized powder

Quality Level

200

technique(s)

affinity chromatography: suitable

matrix

cross-linked 4% beaded agarose

matrix active group

cyanate or related structures

matrix spacer

1 atom (when ligands are coupled via isourea derivative or related linkage)

swelling

1 g swells to 2.5-4.5 mL

capacity

≥10 mg/mL binding capacity (BSA)(via amino groups)

suitability

suitable for chromatography

storage temp.

−20°C

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Related Categories

Application

Cyanogen bromide-activated Agarose is lyophilized powder stabilized with lactose used in affinity chromatography, protein chromatography, protein interactions, antibody labeling, antibody modification and attaching antibodies to agarose beads.

Physical form

Lyophilized powder stabilized with lactose

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000393638
0000375512
0000393638
0000324754
0000324753

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N A Morjana et al.
Protein expression and purification, 5(2), 144-148 (1994-04-01)
Protein disulfide isomerase (PDI) catalyzes the formation and rearrangement of disulfide bonds during protein folding. PDI coupled to cyanogen bromide-activated agarose retains its catalytic activity, and a column of this material increases both the rate and the yield for folding
Mi Young Cha et al.
Bioconjugate chemistry, 22(1), 88-94 (2010-12-15)
We developed fluorescent biosensor systems that are either general or selective to fluoroquinolone antibiotics by using a single-chain variable-fragment (scFv) as a recognition element. The selectivity of these biosensors to fluoroquinolone antibiotics was rationally tuned through the structural modification on
R A Gilissen et al.
Biochemical pharmacology, 43(12), 2661-2663 (1992-06-23)
A method for the covalent binding of rat liver UDP-glucuronosyltransferase to a cyanogen bromide-activated agarose matrix is described. The rat liver microsomal fraction was solubilized with 8 mM 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS); 90% of the microsomal protein was solubilized. Some 50-60% of
Supported Planar Bilayers for Study of the Immunological Synapse.
Dustin, M.L., et al.
Current Protocols in Immunology, 76, 18-18 (2007)
DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA-Protein Interactions <I>In Vivo</I>.
Xiao, R., and Moore, D.D.
Current Protocols in Molecular Biology, 94, 21-21 (2011)
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Questions

1–4 of 4 Questions  

  1. What is the difference between Product C9210, Cyanogen bromide and Product C9142, Cyanogen bromide-activated-Sepharose® 4B?

    1 answer

    1. Sigma-Aldrich produces product C9210, while C9142 is produced by GE Healthcare.  In addition, C9210 is a cross-linked resin and C9142 is not.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. When using Product C9210, Cyanogen bromide, why must amine containing reagents be avoided during coupling?

    1 answer

    1. The ligand is attached to the resin via an amine group. If an amine-containing buffer is used during coupling, you will greatly reduce the amount of ligand that can attach.

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  4. What do you recommend to use as a negative control for Product C9210, Cyanogen bromide?

    1 answer

    1. The control for this product is Product No. CL4B200 (Sepharose CL-4B).

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