Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

B9643

Supelco

Bicinchoninic Acid solution

Synonym(s):

BCA

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352106
NACRES:
NA.32
Pricing and availability is not currently available.

form

liquid

Quality Level

200

suitability

suitable for determination of protein concentration

General description

Protein determination is one of the most common operations performed in biochemical research. Bicinchoninic acid solution is used in the bicinchoninic acid (BCA) assay, which is equivalent to the Lowry procedure. Both rely on the formation of a Cu2+-protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to the protein present. The BCA assay is more sensitive and applicable than either biuret or Lowry procedures.

Application

Bicinchoninic Acid solution (Bicinchoninic acid protein assay kit) is used :
  • For the determination of total protein content in the process of quantifying bromelain, extracted from the pineapple stem residues using aqueous micellar two-phase systems composed of ionic liquids as co-surfactants.[1]
  • For the determination of bovine serum albumin (BSA) in biomimetic calcium phosphate coating co-precipitated on titanium alloy plates.[2]

Features and Benefits

  • It is easy to use.
  • The color complex is stable.
  • There is less susceptibility to detergents.
  • It is applicable over a broad range of protein concentrations.

Packaging

Also available as part of the Bicinchoninic Acid Kit for Protein Determination (BCA-1).

Suitability

This product is commonly used for protein determination by the Bicinchoninic Acid (BCA) method.

related product

Product No.
Description
Pricing

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SHBD1781V
SLCK3724
SLCG8086
SLCD5350
SLCB6552

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Diego García-Ayuso et al.
Experimental eye research, 161, 10-16 (2017-05-30)
In this work we study the effects of an acute light-induced retinal degeneration on the population of melanopsin positive retinal ganglion cells (m
Biomimetic coprecipitation of calcium phosphate and bovine serum albumin on titanium alloy
Liu Y, et al.
Journal of Biomedical Materials Research, 57(3), 327-335 (2001)
Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants
Vicente AF, et al.
Process Biochemistry (Oxford, United Kingdom), 51(4), 528-534 (2016)
Shabnam Khatibi et al.
Growth factors (Chur, Switzerland), 35(2-3), 100-124 (2017-09-28)
Mathematical models for TGF-β and IL-6 signalling have been linked, providing a platform for analyzing the crosstalk between the systems. An integrated IL-6:TGF-β model was developed via a reduced set of reaction equations which incorporate both feedback loops and appropriate
Zhenhua Ma et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 216, 29-37 (2018-11-12)
This study explored the impact of fishmeal replacement by commercial soybean meal (SM) and EnzoMeal (EZM) on Asian seabass Lates calcarifer growth performance using six diets. The six diets comprised two sources of plant proteins with three levels each, including
View All Related Papers

Protocols

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Related Content

Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service