Product 84655 is not suitability tested for the demonstration of aldehydes (PAS positive substances) in tissue sections. The formulation would suggest the product is intended for use in demonstrating aldehydes in solution - not in tissue sections. The recommended product for demonstrating PAS positive substances in tissue sections is 3952015. A kit is offered as product 395B-1KT.
84655
Schiff′s reagent for aldehydes
for microscopy
Synonym(s):
Fuchsin sulfurous acid
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About This Item
UNSPSC Code:
12352300
NACRES:
NA.21
grade:
for microscopy
form:
liquid
Pricing and availability is not currently available.
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General description
Schiff′s reagent is used for the qualitative or quantitative assay of carbohydrates. Periodic acid-Schiff′s reagent (PAS) stain is broadly applicable in histology for visualizing glycogen, glycoproteins, mucins, and other polysaccharides in tissue and cells.
Application
Schiff′s reagent for aldehydes has been used:
- in microtiter PAS assay to quantify the amount of secreted mucin in Vibrio cholera infected water[1][2]
- to stain the colonies of Clostridium perfringens in PGY agar plates to identify plasmalogen-deficient mutants[3]
- in periodic acid-Schiff′s reagent assay to quantify carbohydrates in a microtiter plate format[4]
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Carc. 1B - Met. Corr. 1
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Michelle Kilcoyne et al.
Analytical biochemistry, 416(1), 18-26 (2011-05-31)
Microtiter plate colorimetric assays are widely used for analysis of carbohydrates and glycoconjugates. However, mucins are often not easily detected, as they have low neutral sugar content. We have adapted and optimised the periodic acid-Schiff's reagent (PAS) staining for microtiter
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ACS chemical biology, 16(1), 6-13 (2020-12-23)
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Traditionally cultured monolayers of primary human hepatocytes (PHHs) deteriorate within days and thereby become unsuitable for drug-related studies. PHH spheroids (3D PHHs) maintain liver functions for weeks, but are considerably more demanding. Recently, a chemical-based approach (5C PHHs) succeeded in
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Is Product 84655, Schiff’s reagent for aldehydes, suitable for staining tissue sections for PAS positive substances?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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