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Sigma-Aldrich

Percoll®

colloidal silica coated with polyvinylpyrrolidone

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About This Item

MDL number:
UNSPSC Code:
12352200
Pricing and availability is not currently available.

storage temp.

2-8°C

Application

Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used in cell separation and organelle isolation.
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.

Other Notes

A unique density gradient medium designed to separate cells, subcellular particles and larger viruses under mild conditions. Review[1]; Separation of inner and outer membrane vesicles from E.coli[2]

Legal Information

Percoll is a registered trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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S Morein et al.
Analytical biochemistry, 216(1), 47-51 (1994-01-01)
A rapid and simple method for separating and isolating the inner and outer membranes of Escherichia coli is described. Membrane vesicles were prepared either by passing the bacteria through a French press or by conversion of the cells to spheroplasts
Density gradients prepared from colloidal silica particles coated by polyvinylpyrrolidone (Percoll).
H Pertoft et al.
Analytical biochemistry, 88(1), 271-282 (1978-07-15)
Yanping P Chen et al.
The Journal of eukaryotic microbiology, 56(2), 142-147 (2009-05-22)
Nosema ceranae, a microsporidian parasite originally described from Apis cerana, has been found to infect Apis melllifera and is highly pathogenic to its new host. In the present study, data on the ultrastructure of N. ceranae, presence of N. ceranae-specific
Jieli Chen et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 29(5), 911-920 (2009-02-19)
We tested the hypothesis that Niaspan (a prolonged release formulation of niacin) increases tumor necrosis factor-alpha-converting enzyme (TACE) expression and Notch signaling activity and promotes arteriogenesis after stroke. Rats were subjected to middle cerebral artery occlusion and were treated with
Audrey Caro et al.
Applied and environmental microbiology, 75(10), 3304-3313 (2009-04-07)
The bivalve Codakia orbicularis, hosting sulfur-oxidizing gill endosymbionts, was starved (in artificial seawater filtered through a 0.22-mum-pore-size membrane) for a long-term experiment (4 months). The effects of starvation were observed using transmission electron microscopy, fluorescence in situ hybridization and catalyzed

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