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46955

Sigma-Aldrich

Fluorescein

for fluorescence, free acid

Synonym(s):

Acid Yellow 73

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About This Item

Empirical Formula (Hill Notation):
C20H12O5
CAS Number:
2321-07-5
Molecular Weight:
332.31
Colour Index Number:
45350
Beilstein:
94324
EC Number:
219-031-8
MDL number:
MFCD00005050
UNSPSC Code:
12171500
PubChem Substance ID:
329757275
NACRES:
NA.32
Pricing and availability is not currently available.

grade

for fluorescence

Quality Level

100

technique(s)

titration: suitable

fluorescence

λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H

InChI key

GNBHRKFJIUUOQI-UHFFFAOYSA-N

Gene Information

human ... PTPN1(5770) , PTPRF(5792)

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General description

Fluorescein dye is the most commonly used fluorescent probe.[1] It is used to stain epithelial defects and analyse the integrity of corneal epithelium. Fluorescein is used in the diagnosis of corneal perforation.[2] Oral or intravenous fluorescein is used in fluorescein angiography to detect diabetic retinopathy and choroidal neovascular membrane.[3]

Application

Fluorescein has been used:
  • in the permeability assay of Caco-2 cells[4][5]
  • to test its suitability to serve as lifetime fluorescence standards[6]
  • as a fluorophore for the fast recovery of fluorescence peaks and fluorophore concentration in the presence of hemoglobin[7]
  • as a fluorescent probe in oxygen radical absorbance capacity (ORAC) assay[8]

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCBV9534
BCBV5681
BCBV8435
BCBQ8001V
BCBP5200V

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Absorption and fluorescence properties of fluorescein
Sjoback R, et al.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 51(6), L7-L21 (1995)
Rapid measurement of total antioxidant capacity in plants
Gillespie KM, et al.
Nature Protocols, 2(4), 867-867 (2007)
Ocular health assessment
Prokopich CL, et al.
Clinical Pediatric Nephrology, Second Edition, 221-318 (2007)
Lucie Vobecká et al.
International journal of molecular sciences, 20(14) (2019-07-25)
Electrodialysis and electrodeionization are separation processes whose performance depends on the quality and properties of ion-exchange membranes. One of the features that largely affects these properties is heterogeneity of the membranes both on the macroscopic and microscopic level. Macroscopic heterogeneity
Microfluidic gut-on-a-chip with three-dimensional villi structure
Shim KY, et al.
Biomedical Microdevices, 19(2), 37-37 (2017)
View All Related Papers

Articles

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

Fluorescence Quenching Microscopy: Imaging Two-Dimensional Materials

Developed in the last several years, fluorescence quenching microscopy (FQM) has enabled rapid, inexpensive, and high-fidelity visualization of two-dimensional (2D) materials such as graphene-based sheets and MoS2.

Questions

1–7 of 7 Questions  

  1. What is a free acid?

    1 answer

    1. A free acid is the acidic form of a substance in which all acidic groups are fully protonated.

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  2. What is the size in kDa of this Fluorescein?

    1 answer

    1. This product has a molecular weight size of 332.32 g/mol or 332.32 daltons. The kDa unit designates kilodaltons, 1 kDa is equivalent to 1000 daltons.

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  3. How long can this product be kept once it's made into a solution? If it can be kept more than 1 day; what are the storage conditions? Thank you

    1 answer

    1. The stability of this product is not determined. However, various sources state that this product is stable in solvent at -80°C for 6 months; at -20°C for 1 month (protect from light).

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  4. What is the dye content of Product 46955, Fluorescein?

    1 answer

    1. According to our supplier, the dye content of pure Fluorescein is a minimum of 90%. We do not test the purity of this product since it is not one of our specifications.

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  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  6. How should Product 46955, Fluorescein, be stored?

    1 answer

    1. We recommend storing this product at room temperature.

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  7. What is Product 46955, Fluorescein, soluble in?

    1 answer

    1. According to the chemicals encyclopedia published by the Royal Society of Chemistry : 13th Edition, Fluorescein is soluble in hot alcohol and glacial acetic acid. Also, it is soluble in alkali hydroxides or carbonates. This product is insoluble in water, benzene, chloroform and ether.

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